doi:10.1016/j.diagmicrobio.2008.02.010 
Copyright © 2008 Published by Elsevier Inc.
Characterization of ST80 Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus clone in Tunisia
Mouna Ben Nejmaa, Maha Mastourib, Besma Bel Hadj Jrada and Mohamed Noura, 
, 
aHigh Institute of Biotechnology, Medical Biotechnology, Monastir, UR-09-11, Tunisia 5000, Tunisia
bLaboratory of Microbiology, CHU, F. Bourguiba, Monastir, Tunisia 5000, Tunisia
Received 5 December 2007;
accepted 15 February 2008.
Available online 3 April 2008.
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Abstract
The spread of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has been reported in communities worldwide. In this study, we characterized 64 Tunisian CA-MRSA by agr typing, polymerase chain reaction assay for 20 virulence genes, staphylococcal chromosomal cassette mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and protein A gene (spa) typing. All our isolates were lukS-PV-lukF-PV positive, etd positive, and edin positive. They harbored SCCmec type IV and belonged to agr group 3. PFGE typing showed that our isolates were distributed in 11 different pulsotypes. spa typing and MLST, performed with isolates representative of each PFGE pattern, revealed that all isolates had a unique spa type (t044) and a common sequence type (ST80). The isolates showed susceptibility to the majority of antibiotics, and resistance to kanamycin, erythromycin, and tetracycline, but intermediate resistance to fusidic acid. Full analysis of our results revealed that our isolates were nonmultiresistant and belonged to a single clonal type ST80.
Keywords: MRSA; PVL; PFGE; ST80; Spa typing
Fig. 1. PFGE patterns of SmaI-digested genomic DNA from MRSA isolates. TUN 1–4: pulsotypes of representative Tunisian CA-MRSA strains; NCTC 8325: S. aureus reference strain.
Fig. 2. Schematic representation of the PFGE patterns and corresponding dendrogram, agr type, ST, and spa type of 64 Tunisian PVL-positive CA-MRSA and 4 French MRSA isolates. PFGE types F1–F4 correspond to French isolates. NT = nontypeable.
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