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doi:10.1016/j.cyto.2004.12.018 
Copyright © 2005 Elsevier Ltd All rights reserved.
Measurement of gp130 cytokines – Oncostatin M and IL-6 in gingival crevicular fluid of patients with chronic periodontitis
Received 16 October 2004;
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Abstract
Several proinflammatory cytokines can induce periodontal tissue destruction and are thought to be useful indicators or diagnostic markers for periodontitis. Here, we aimed to investigate whether oncostatin M (OSM) was present in gingival crevicular fluid (GCF) and to clarify the correlation of GCF OSM and interleukin-6 (IL-6) levels with the severity of periodontitis. Sixty-two sites in 14 patients were divided into 4 groups based on probing depth (PD) and bleeding on probing (BOP). GCF was collected using paper strips from clinically health sites (PD ≤ 3 mm, CAL: 1–3 mm, without BOP, n = 31), mildly diseased sites (PD ≤ 3 mm, CAL: 3–5 mm, with BOP, n = 11), moderately diseased sites (PD = 4–6 mm, CAL: 5–8 mm, with BOP, n = 11), and severely diseased sites (PD > 6 mm, CAL: 8–12 mm, with BOP, n = 9). IL-6 and OSM in GCF were quantified by enzyme-linked immunosorbent assay and are expressed as concentrations (pg/ml) and total amounts (pg/site). Correlations of OSM and IL-6 levels with the severity of periodontitis in all groups were determined using Spearman rank correlation (rs). Our results showed that OSM and IL-6 were detected in most GCF samples. The total amounts of OSM and IL-6 were significantly positive correlated with severity of diseased sites (OSM: rs = 0.526, p < 0.01; IL-6: rs = 0.729, p < 0.01). No correlations of OSM or IL-6 concentration in GCF were found with disease severity. OSM and IL-6 levels in GCF were positively correlated to each other when expressed as either concentrations or total amounts (concentrations: r = 0.485, p < 0.01; total amounts r = 0.490, p < 0.01). In conclusion, our findings suggest that IL-6 and OSM may play a role in modulating the inflammatory cascade of chronic periodontitis.
Keywords: Gingival crevicular fluid; Interleukin-6; Oncostatin M; Periodontitis
Article Outline
- 1. Introduction
- 2. Results
- 2.1. Expression of OSM in GCF
- 2.2. Expression of IL-6 in GCF
- 2.3. Correlation of OSM/IL-6 in GCF and disease severity
- 3. Discussion
- 4. Materials and methods
- 4.1. Patient and site selection
- 4.2. Collection of GCF
- 4.3. Determination of IL-6 and OSM
- 4.4. Statistical analysis
- Acknowledgements
- References
