Dynamics of water in and around proteins characterized by <sup>1</sup>H-spin-lattice relaxometry

Robert G. Bryant

doi:10.1016/j.crhy.2010.06.013

Dynamics of water in and around proteins characterized by ¹H-spin-lattice relaxometry
[Caractérisation par relaxométrie spin-réseau des protons de la dynamique de l'eau à l'intérieur et autour des protéines]

Robert G. Bryant ¹

¹ Chemistry Department, University of Virginia, P.O. Box 400319, Charlottesville, VA 22904-4319, USA

Comptes Rendus. Physique, Volume 11 (2010) no. 2, pp. 128-135.

Résumé
Abstract

Les vitesses de relaxation magnétique spin-réseau mesurées en fonction de l'intensité du champ magnétique sur de grandes plages de fréquences de Larmor permettent de suivre la densité spectrale du bruit qui induit la relaxation. Pour les mouvements de l'eau à l'intérieur et autour des systèmes de protéines, la relaxation de spin mesure la dynamique de translation moyenne à l'interface protéine/eau qui est réduit d'un facteur trois par rapport à celle de la solution. Il y a de plus un effet d'anisotropie induit par les mouvements moléculaires venant du volume exclu par la présence de la protéine. L'eau pénètre la protéine également et un très petit nombre de sites d'eau liée qui engendre un fort couplage entre la dynamique de la protéine et la relaxation des protons de l'eau.

Nuclear magnetic spin-lattice relaxation rate constants measured as a function of the magnetic field strength over wide ranges of Larmor frequency map the noise spectrum that drives spin relaxation. For water in and around protein systems, the spin relaxation reports on the average local translational mobility at the interface which is reduced by approximately factor of three from the bulk and there is anisotropy induced in the motions caused by the excluded volume created by the presence of the protein. Water also penetrates the protein and relatively few bound water sites provide a strong coupling between the protein dynamics and the water-proton-spin relaxation.

Publié le : 2010-03-01

DOI : 10.1016/j.crhy.2010.06.013

Keywords: Water, Protein, Spin-lattice relaxation, Diffusion, Relaxation dispersion, Protein dynamics
Mot clés : Eau, Protéine, Relaxation spin-réseau, Diffusion, Dispersion de relaxation, Dynamique des protéines

Affiliations des auteurs :

Robert G. Bryant ¹

¹ Chemistry Department, University of Virginia, P.O. Box 400319, Charlottesville, VA 22904-4319, USA

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Robert G. Bryant. Dynamics of water in and around proteins characterized by ¹H-spin-lattice relaxometry. Comptes Rendus. Physique, Volume 11 (2010) no. 2, pp. 128-135. doi : 10.1016/j.crhy.2010.06.013. https://comptes-rendus.academie-sciences.fr/physique/articles/10.1016/j.crhy.2010.06.013/

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