Fig. 1. Surface pressure–area isotherms of DPPC/p24-1 monolayers containing different peptide concentrations: (—) pure DPPC, 0.05 mol%, 0.03 mol% and 0.5 mol% p24-1 in the monolayer.

Fig. 2. Area ratio between the mixed DPPC/p24-1 and the pure DPPC monolayers, A/A₀ at three pressures: 1 mN/m, 30 mN/m and LE/LC phase transition. Each point represents the average of three experiments.

Fig. 3. Ratio between the dipole moments of the mixed DPPC/p24-1 and the pure DPPC monolayers: 1 mN/m, 30 mN/m and LE/LC phase transition. Each point represents the average of three experiments.

Fig. 4. BAM micrographs obtained for pure DPPC at 10 mN/m (a) and 30 mN/m (c), and a mixed DPPC/p24-1 monolayer with 0.5 mol% at 10 mN/m (b) and 30 mN/m (d). Comparison between the mixed and the pure monolayers is not straightforward because the mixed monolayer is more expanded than the pure one. Nevertheless, one can note that the presence of p24-1 prevents DPPC molecules from aggregating, which is characterized by the smaller domains in (b) and (d). Essentially, the same features are seen in micrographs for mixtures with higher contents of p24-1.

Fig. 5. CD spectra of p24-1 in water (solid line —), mixture of water and methanol (dashed line ---) and in the presence of MLV (dotted line ···).

Fig. 6. Surface pressure–area isotherms of DPPG/p24-1 monolayers containing different peptide concentrations: pure DPPG, 0.1 mol% and 0.5 mol% p24-1 in the monolayer.