Fig. 1. Chemical structures of coralyne (A) and berberine (B) cations.

Fig. 2. Fluorescent response (area counts) vs. mass of n-C₂₂ (A), and n-C₁₆ (B) on a coralyne-impregnated (6 mg L⁻¹) silica gel HPTLC plate. Application volume: 2 μL; development: n-hexane (5 min); λ_ex = 430 nm; emission >450 nm.

Fig. 3. (A) HPTLC-scanning fluorescence chromatograms of n-C₁₂ (● ● ●), n-C₁₆ ( ), n-C₁₈ ( ), n-C₂₂ (● ● ●), n-C₂₄ ( ), and blank ( ) on a coralyne-impregnated (6 mg L⁻¹) silica gel HPTLC plate. (B) Fluorescent responses (area counts) vs. number of C atoms for the above-mentioned alkanes on coralyne-impregnated (a) (6 mg L⁻¹) and berberine-impregnated (b) (60 mg L⁻¹) silica gel HPTLC plates. Application volume: 1 μL; applied mass: 0.8 μg; development: n-hexane (5 min); λ_ex = 410 nm; emission >450 nm.

Fig. 4. (A) HPTLC-scanning fluorescence chromatograms of n-C₁₀OH (● ● ●), n-C₁₂OH ( ), n-C₁₆OH ( ), n-C₁₈OH (● ● ●), n-C₂₂OH ( ), and blank ( ) on a coralyne-impregnated (6 mg L⁻¹) silica gel HPTLC plate. (B) Fluorescent responses (area counts) vs. number of C atoms for the above-mentioned alcohols on coralyne-impregnated (a) (6 mg L⁻¹) and berberine-impregnated (b) (60 mg L⁻¹) silica gel HPTLC plates. Application volume: 1 μL; applied mass: 0.8 μg; development: dichloromethane (5 min); λ_ex = 430 nm; emission >450 nm.

Fig. 5. (A) HPTLC chromatogram of rifampicine (dotted line: 1 μg; continuous line: 3 μg) on a coralyne-impregnated (6 mg L⁻¹) silica gel HPTLC plate, and detected by UV at 254 nm. (B) Corresponding HPTLC chromatogram, under the same conditions, detected by fluorescence scanning (λ_ex = 430 nm; emission > 450 nm). Application volume: 1 μL; development: methanol (5 min).

Fig. 6. HPTLC-scanning fluorescence chromatograms of a mixture of neutral lipids on a coralyne-impregnated (6 mg L⁻¹) silica gel HPTLC plate: (A) cholesterol (0.60 μg); (B) cholesteryloleate (0.60 μg); (C) oleic acid (0.81 μg); (D) oleic acid methyl ester (1.12 μg); (E) triolein (0.56 μg). Development: see Section 2; application volume: 1 μL; λ_ex = 430 nm; emission > 450 nm.

Fig. 7. Fluorescent response (area counts) vs. polarizability (α) of alkanes (A) or alcohols (B) on coralyne-impregnated (a) (6 mg L⁻¹) and berberine-impregnated (b) (60 mg L⁻¹) silica gel HPTLC plates. Conditions: see Fig. 3 for alkanes, and Fig. 4 for alcohols.

Fluorescent response factors (area mol⁻¹) of n-docosane, 1-docosanol, and 1-bromodocosane applied on coralyne-impregnated (6 mg L⁻¹) and berberine-impregnated (60 mg L⁻¹) HPTLC silica gel plates (λ_ex = 365 nm for berberine, and 410 nm for coralyne)

Fluorescent response factors (area mol⁻¹) of n-hexadecane, 1-hexadecanol, and 1-bromohexadecane applied on coralyne-impregnated (6 mg L⁻¹) HPTLC silica gel plates (λ_ex = 410 nm), and values of dielectric constant () for these compounds at the same temperature

Parameters of linearity of ion-induced dipole model, on coralyne (6 mg L⁻¹) and berberine (60 mg L⁻¹)-impregnated silica gel HPTLC plates

Analyte mass: 0.8 μg; λ_ex = 410 nm; emission > 450 nm.