Cell
Volume 129, Issue 7, 29 June 2007, Pages 1401-1414
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A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing

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Summary

MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.

MOLENEURO
MOLIMMUNO
RNA

Cited by (0)

24

Present address: Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01605, USA.

25

Present address: IBM-CNRS, 12, rue du Général Zimmer, 678084 Strasbourg Cedex, France.

26

Present address: Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, NY 11724, USA.

27

Present address: Laboratory of Developmental Neurogenetics, Rockefeller University, New York, NY 10021, USA.

28

Present address: Department of Pediatric Oncology, Hematology and Immunology, Heinrich Heine University Medical Center, 40225 Düsseldorf, Germany.

29

Present address: Computational Biology Group, Department of Medical Genetics, University of Lausanne, 1005 Lausanne, Switzerland.