Elsevier

Chinese Chemical Letters

Volume 28, Issue 10, October 2017, Pages 1991-1993
Chinese Chemical Letters

Communication
A α-KA fluorescent probe for discrimination of blood cancer serum

https://doi.org/10.1016/j.cclet.2017.08.054Get rights and content

Abstract

α-Ketoglutaric acid (α-KA) is an important metabolic intermediate in tricarboxylic acid circle in our body. The mutations of isocitrate dehydrogenase-1 (IDH1) and isocitrate dehydrogenase-2 (IDH2), however, will lead to the transformation of α-KA into 2-hydroxyglutarate (2-HG), which is confirmed to closely related to actue myeloid leukemia (AML). Therefore it is of great significance to detect α-KA level changes in serum. In this paper, a fluorescent “off-on” probe CH for α-KA was designed based on naphthalimide fluorophore by introducing a hydrazine group for α-KA recognition and a long alkyl amino chain to enhance PET efficiency and water solubility. Cetyltrimethyl ammonium bromide (CTAB) was added toform self-assembly micelles for accelerating the recognition process. CH shows a 28-fold fluorescence enhancement ((I  I0)/I0 at 550 nm) over other biological species by optimizing the chemical recognition process of CH with α-KA. Significantly, CH was successfully applied for thefluorescence discrimination of all kinds of blood cancer serum samples. This work would provide a potential method that is quick and convenient for sensing α-KA and may promote fluorescence detection in clinical diagnosis.

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Probe CH can quickly detect α-KA in human serum with micelles of CTAB and could differentiate cancer from normal blood serum.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Nos. 21576037, 21422601, 21406028, 21421005), NSFC-Liaoning United Fund (No. U1608222).

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