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doi:10.1016/j.cbi.2006.09.011 
Copyright © 2006 Elsevier Ireland Ltd All rights reserved.
Suppressive effect of Withania somnifera root powder on experimental gouty arthritis: An in vivo and in vitro study
Received 31 March 2006;
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Abstract
The effect of Withania somnifera L. Dunal root powder on paw volume and serum lysosomal enzyme activities was investigated in monosodium urate crystal-induced rats. The levels of β-glucuronidase and lactate dehydrogenase were also measured in monosodium urate crystal incubated polymorphonuclear leucocytes (PMNL). A significant increase in the level of paw volume and serum lysosomal enzymes was observed in monosodium urate crystal-induced rats. The increased β-glucuronidase and lactate dehydrogenase level were observed in untreated monosodium urate crystal incubated polymorphonuclear leucocytes. On treatment with the W. somnifera root powder (500/1000 mg/kg body weight), the above changes were reverted back to near normal levels. W. somnifera also showed potent analgesic and antipyretic effect with the absence of gastric damage at different dose levels in experimental rats. For comparison purpose, non-steroidal anti-inflammatory drug (NSAID) indomethacin was used as a standard. These results provide evidence for the suppressive effect of W. somnifera root powder by retarding amplification and propagation of the inflammatory response without causing any gastric damage.
Keywords: Withania somnifera; Indomethacin; Gouty arthritis
Article Outline
- 1. Introduction
- 2. Materials and methods
- 2.1. Drug
- 2.2. Synthesis of monosodium urate crystals
- 2.3. Animals
- 2.4. Monosodium urate crystal-induced inflammation in rats
- 2.4.1. Assessment of inflammation
- 2.5. In vitro studies (monosodium urate crystal–PMNL cell interaction)
- 2.6. Test of the analgesic activity of W. somnifera
- 2.7. Test of the antipyretic activity of W. somnifera
- 2.8. Test of the ulcerogenic activity of W. somnifera
- 2.9. Statistical analysis
- 3. Results
- 3.1. Effect of W. somnifera root powder and indomethacin on in vivo studies
- 3.2. Effect of W. somnifera root powder and indomethacin on in vitro studies
- 3.3. Analgesic activity of W. somnifera root powder
- 3.4. Antipyretic effect of W. somnifera root powder
- 3.5. Ulcerogenic effect of W. somnifera root powder
- 4. Discussion
- References
