NoteStructural characterization of a water-soluble β-(1→6)-linked d-glucan isolated from the hot water extract of an edible mushroom, Agaricus bitorquis
Graphical abstract
→6)-β-d-Glcp-(1→
Section snippets
General methods
Optical rotation was measured on a Jasco Polarimeter model P-1020 at 25 °C. All GLC–MS experiments were carried out in a Hewlett–Packard 5970 MSD instrument using HP-5 fused silica capillary column. The program was isothermal at 150 °C; hold time 2 min, with a temperature gradient of 4 °C min−1 up to a final temperature of 200 °C. The molecular weight of the polysaccharide was determined as described previously.30, 31, 32, 33, 34 For monosaccharide analysis, the polysaccharide sample (2.5 mg) was
Acknowledgments
The authors are grateful to Professor S. Roy, Director, IICB, Dr. A. K. Sen (Jr.), IICB and Dr. S. Lahiri, IACS, Kolkatta, for providing instrumental facilities. Mr. Barun Majumder of Bose Institute, Kolkata, is acknowledged for preparing NMR spectra. DST, Govt of India is acknowledged for sanctioning a project (Ref. No.: SR/S1/OC-52/2006 dated 19/02/2007). One of the authors (C.K.N.) is grateful to UGC, New Delhi for offering junior research fellowship.
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Recent advances in the biosynthesis of fungal glucan structural diversity
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2019, International Journal of Biological MacromoleculesCitation Excerpt :These results confirmed that CMS is →6)-β-D-Glcp(1→ linked glucan, with α-D-Glcp(1→ at the non-reducing end (Fig. 4C). NMR data of CMS is consistent with a previously reported →6)-β-D-Glcp(1→ linked glucan isolated from an edible mushroom, Agarius bitorquis [36]. The NMR results of α-D-Glcp(1→ at the non-reducing end and the β-D-Glcp(1→ at the reducing end were consistent with a previous report [37].
Water-insoluble glucans from the edible fungus Ramaria botrytis
2014, Bioactive Carbohydrates and Dietary FibreCitation Excerpt :Several glucans have been reported from various sources using different procedures (Bohn & BeMiller, 1995; Kollar et al., 1997). The glucans in mushrooms are present mostly as linear β-(1→3)-(Misaki & Kakuta, 1995; Ojha, Chandra, Ghosh, & Islam, 2010), β-(1→6)-(Nandan et al., 2008; Sarkar et al., 2012) and non linear form with β-(1→3) backbone branched at O-6 (Mizuno et al., 1990; Rout, Mondal, Chakraborty, & Islam, 2008), and β-(1→6) backbone branched at O-3 (Maji et al., 2012; Sen et al., 2013). The β-d-glucans are biologically important for their outstanding ability to enhance and stimulate the immune systems of human (Blascheck, Kasbauer, Kraus, & Franz, 1992; Kiho, Sakushima, Wang, Nagai, & Ukai, 1991; Kulicke, Lettau, & Thielking, 1997) and are thus regarded as typical biological response modifiers (BRMs).
Structural characterization of an immunoenhancing glucan isolated from a mushroom Macrolepiota dolichaula
2013, International Journal of Biological MacromoleculesCitation Excerpt :Now days, mushrooms have drawn the attention of immunobiologist and pharmacologist for their immunostimulatory, antitumor, and antioxidant properties [1–3]. The glucans in mushroom are present mostly as linear β-(1 → 3)- [4–6], β-(1 → 6)- [7–9] and non linear with β-(1 → 3) backbone branched at O-6 [10,11], and β-(1 → 6) backbone branched at O-3 [12,13]. The genus Macrolepiota, family Agaricaceae was first established by Singer [14].
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2013, Carbohydrate PolymersStructural diversity of fungal glucans
2013, Carbohydrate PolymersCitation Excerpt :Inter-unit 13C–1H correlations obtained from the HMBC experiment also confirmed (1→6)-connection between β-d-Glcp units. Cross peaks were found between H-1 (δ 4.50) and C-6 (δ 69.2), and between C-1 (δ 103.4) and H-6 (δ 4.20 and 3.84) of neighbour residues (Nandan et al., 2008). Ukawa, Ito, and Hisamatsu (2000) described three polysaccharide fractions isolated from a hot-water extract of Lyophyllum decastes basidiocarps.