Structural analysis of a water-soluble glucan (Fr.I) of an edible mushroom, Pleurotus sajor-caju

doi:10.1016/j.carres.2007.08.012

Carbohydrate Research

Volume 342, Issue 17, 10 December 2007, Pages 2670-2675

https://doi.org/10.1016/j.carres.2007.08.012 Get rights and content

Abstract

A water-soluble glucan was obtained from the fruit bodies of an edible mushroom, Pleurotus sajor-caju, by hot water extraction, ethanol precipitation, dialysis, and Sepharose 6B gel filtration. On the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR studies (¹H, ¹³C, DQF-COSY, TOCSY, NOESY, and HSQC), the structure of the repeating unit of the glucan is determined as

Graphical abstract

Section snippets

Isolation and purification of the polysaccharide

The polysaccharide, Fr.I, was extracted from the fruit body of P. sajor-caju as described earlier.³ From 1 kg of fruits, 584.5 mg of Fr.I was obtained.

Determination of molecular weight

The molecular weight of Fr.I was determined by a gel-chromatographic technique. Standard dextrans T-200, T-70, and T-40 were passed through a Sepharose 6B column, and then the elution volumes were plotted against the logarithms of their respective molecular weights. The elution volume of Fr.I was then plotted in the same graph, and the molecular

Acknowledgements

The authors are grateful to Professor S. Roy, Director, IICB, Kolkata for providing NMR facilities, and Mr. Barun Majumder for technical assistance. The authors are thankful to Dr. R. N. Singh, and Mr. S. Goswami, Chemgen Pharma International, Kolkata for giving instrumental facility.

References (26)

M. Pramanik et al.
Carbohydr. Res.
(2005)
Y. Yoshioka et al.
Carbohydr. Res.
(1985)
D. Rout et al.
Carbohydr. Res.
(2005)
C. Hara et al.
Carbohydr. Res.
(1982)
O.H. Lowry et al.
Biol. Chem.
(1951)
G.J. Gerwig et al.
Carbohydr. Res.
(1979)
I. Ciucanu et al.
Carbohydr. Res.
(1984)
Y. Dong et al.
The Edible Mushrooms in China
(1988)
C. Zhuang et al.
Biosci. Biotechnol. Biochem.
(1993)
D.M. Soden et al.
Microbiology
(2001)

D.M. Soden et al.

Microbiology

(2002)

H.J. Mrjana et al.

Serb. Chem. Soc.

(1988)

J. Zhang et al.

Biosci. Biotechnol. Biochem.

(1994)

Cited by (45)

Sulfated modification, characterization and bioactivities of an acidic polysaccharide fraction from an edible mushroom Pleurotus eous (Berk.) Sacc.
2021, Heliyon
Citation Excerpt :
Edible variety of mushrooms of the species Pleurotus such as P. florida, P. ostreatus, P. djamor, P. citrinopileatus, P. sajor-caju and P. eous are considered non-toxic. Polysaccharide extraction and its biological properties from Pleurotus sajor-caju (Seedevi et al., 2019; Rout et al., 2008), Pleurotus florida (Pramanik et al., 2007), Pleurotus ostreatus (Silva et al., 2012), Pleurotus eryngii (Xu et al., 2016) and Pleurotus tuber-regium (Wu et al., 2013) have been reported. Pleurotus eous (Berk.),
The acidic fraction (P3a) of Pleurotus eous was successfully sulfated by sulphur trioxide-pyridine complex method. The effect of sulfate modification (SP3a) on the structure, physicochemical properties and in vitro biological activity of P3 was studied. The structural characteristics were established by UV absorption, FT-IR, HPGPC and GC-MS. Biological studies were carried out, such as in vitro antioxidant, anticoagulant, anti-tumour and antibacterial activities. The sulfation process changed its physicochemical and biological characteristics. Compared with P3a, the molecular weight of SP3a is reduced. P3a and SP3a are composed of galactose, xylose, arabinose with different molar percentages. Sulfated derivatives have strong antioxidant and anticoagulant properties. Compared with P3a, SP3a showed obvious cytotoxicity to Jurkat and HeLa cells. SP3a showed a higher inhibition zone for Gram-positive and Gram-negative bacteria. This article demonstrates that sulfation is an effective way to enhance biological activity, especially SP3a is a promising candidate for bioactive macromolecules and has great potential for industrial and biomedical applications.
Polysaccharides of mushroom Pleurotus spp.: New extraction techniques, biological activities and development of new technologies
2020, Carbohydrate Polymers
The biodiversity of mushrooms Pleurotus spp. is impressive due to its complexity and diversity related to the composition of chemical structures such as polysaccharides, glycoproteins and secondary metabolites such as alkaloids, flavonoids and betalains. Recent studies of polysaccharides and their structural elucidation have helped to direct research and development of technologies related to pharmacological action, production of bioactive foods and application of new, more sophisticated extraction tools. The diversity of bioactivities related to these biopolymers, their mechanisms and routes of action are constant focus of researches. The elucidation of bioactivities has helped to formulate new vaccines and targeted drugs. In this context, in terms of polysaccharides and the diversity of mushrooms Pleurotus spp., this review seeks to revisit the genus, making an updated approach on the recent discoveries of polysaccharides, new extraction techniques and bioactivities, emphasising on their mechanisms and routes in order to update the reader on the recent technologies related to these polymers.
Structural studies of a water insoluble β-glucan from Pleurotus djamor and its cytotoxic effect against PA1, ovarian carcinoma cells
2019, Carbohydrate Polymers
A water insoluble β-glucan (PS), with molecular mass ∼9.16 × 10⁴ Da was isolated from the 4% alkaline extract of an edible mushroom, Pleurotus djamor and found to consist of (1→3)-β-d-glucopyranosyl moiety. The structure of the PS was elucidated on the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments (¹H, ¹³C, DQF-COSY, DEPT-135, and HSQC). The structure of the repeating unit of the polysaccharide was established as:
→3)-β-d-Glcp-(1→.
The water insoluble β-glucan showed cytotoxic effect against PA1 cells, where˜50% population was destroyed at 100 μg/mL concentration, and almost all cells at 250 μg/mL concentration. The wound healing assay showed significant anticarcinogenic effect against ovarian carcinoma PA1 cells after 48 h of treatment.
Isolation, molecular characterization and antioxidant activity of a water-soluble polysaccharide extracted from the fruiting body of Termitornyces albuminosus (Berk.) Heim
2019, International Journal of Biological Macromolecules
A water-soluble polysaccharide WSP1 was extracted from the fruiting body of Termitornyces albuminosus. Its molecular weight, monosaccharide composition and molecular structure were determined by GPC, GC–MS, UV spectroscopy, FT-IR spectroscopy, methylation analysis, NMR (1D and 2D) and AFM. Moreover, the antioxidant activity of WSP1 was evaluated in vitro by the tests of reducing power, scavenging ability on DPPH radical and hydroxyl radical, and chelating ability on ferrous ion. The results indicated that the molecular weight of WSP1 was 9 kDa, and it was mainly composed of fucose and galactose in a molar ratio of 1:3.09. Based on monosaccharide composition, methylation analysis and NMR, the possible repeating unit of WSP1 was presented as follows:
→2-α-l-Fucp-1→ (6-α-d-Galp-1)₃→.
The antioxidant assay revealed that, in the concentration range tested in this experiment, WSP1 had strong scavenging ability on DPPH radical, suggesting that WSP1 could be potentially used as a powerful radical scavenger.
Studies on structure and antioxidant properties of a heteroglycan isolated from wild edible mushroom Lentinus sajor-caju
2018, International Journal of Biological Macromolecules
A water-soluble heteroglycan (PS-I) isolated from the aqueous extract of a wild edible mushroom Lentinus sajor-caju showed average molecular weight ∼1.79 × 10⁵ Da. The structure of the polysaccharide was determined using chemical and 1D/2D NMR experiments. Acid hydrolysis indicated the presence of d-glucose, d-galactose, d-mannose, and l-fucose in a molar ratio of nearly 4:4:1:1 respectively. The presence of terminal Fucp, terminal Galp, (1 → 3)-Glcp, (1 → 6)-Galp, (1 → 6)-Glcp, (1 → 4,6)-Galp, and (1 → 2,4)-Manp moieties were established from methylation analysis. The chemical and NMR analyses indicated that the PS-I was a heteroglycan composed of a repeating unit with backbone chain of three (1 → 6)-α-d-galactopyranosyl residues, two (1 → 6)-β-d-glucopyranosyl residues, one (1 → 4)-α-d-mannopyranosyl residue, and two (1 → 3)-β-d-glucopyranosyl residues where one (1 → 6)-α-d-galactopyranosyl residue was branched at O-4 position with terminal α-l-fucopyranosyl residue and (1 → 4)-α-d-mannopyranosyl residue was branched at O-2 position with terminal α-d-galactopyranosyl residue and the structure was proposed as;
The PS-I is a moderate antioxidant compound which showed DPPH radical scavenging activity, hydroxyl radical scavenging activity, ABTS radical scavenging property, reducing power, and ferrous ion chelating ability.
Effective composting of empty fruit bunches using potential Trichoderma strains
2017, Biotechnology Reports
Oil palm fibres are easy to degrade, eco-friendly in nature and once composted, they can be categorized under nutrient-enriched biocompost. Biocompost is not only a good biofertilizer but also a good biocontrol agent against soil-borne pathogens. In this research, experimental works on the composting of empty fruit bunches (EFB) from the oil palm industry were conducted using two potential Trichoderma strains. Analysis of pH initially found the soils to be slightly acidic. However, after composting, the soils were found to be alkaline. Trichoderma propagules increased by 72% in the soils compared to other fungi. Soil electrical conductivity was found to be 50.40 μS/cm for compost A, 42.10 μS/cm for compost B and 40.11 μS/cm for the control. The highest C:N ratio was obtained for compost A at 3.33, followed by compost B at 2.79, and then the control at 1.55. The highest percentages of nitrogen (N), phosphorus (P), and potassium (K) were found in compost A (0.91:2.13:6.68), which was followed by compost B (0.46:0.83:5.85) and then the control (0.32:0.26:5.76). Thus, the biocomposting of oil palm fibres shows great potential for enhancing soil micronutrient, plant growth performance, and crop yield production.

View all citing articles on Scopus

View full text

NoteStructural analysis of a water-soluble glucan (Fr.I) of an edible mushroom, Pleurotus sajor-caju

Abstract

Graphical abstract

Section snippets

Isolation and purification of the polysaccharide

Determination of molecular weight

Acknowledgements

Carbohydr. Res.

Carbohydr. Res.

Carbohydr. Res.

Carbohydr. Res.

Biol. Chem.

Carbohydr. Res.

Carbohydr. Res.

The Edible Mushrooms in China

Biosci. Biotechnol. Biochem.

Microbiology

Microbiology

Serb. Chem. Soc.

Biosci. Biotechnol. Biochem.

Note
Structural analysis of a water-soluble glucan (Fr.I) of an edible mushroom, Pleurotus sajor-caju