Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein

doi:10.1016/j.bmcl.2009.02.084

Bioorganic & Medicinal Chemistry Letters

Volume 19, Issue 8, 15 April 2009, Pages 2285-2288

https://doi.org/10.1016/j.bmcl.2009.02.084 Get rights and content

Abstract

We report here the development of a novel fluorescein-based probe which shows selective fluorescence enhancement on binding to a hexahistidine-tagged protein. No fluorescence change was observed with untagged protein. This probe is excitable with visible light and is considered to be suitable for use in biological applications.

Graphical abstract

A novel fluorescein-based probe is reported. This molecule shows selective fluorescence enhancement on binding to a hexahistidine tag on the protein surface.

Section snippets

Acknowledgments

This work was supported in part by a Grant-in-Aid for University and Society Collaboration (12793009) to T.H., and a Grant-in-Aid for Young Scientists (B) (19790094) to N.U. from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, and by a Grant-in-Aid for Research in Nagoya City University to N.U. We thank Professor H. Sajiki and Dr. Y. Monguchi (Gifu Pharmaceutical University) for HRMS-FAB analysis.

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Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein

Abstract

Graphical abstract

Section snippets

Acknowledgments

J. Am. Chem. Soc.

Nat. Methods

Proc. Natl. Acad. Sci. U.S.A.

J. Am. Chem. Soc.

Chem. Biol.

Proc. Natl. Acad. Sci. U.S.A.

ACS Chem. Biol.

Anal. Chem.

Bioconjugate Techniques

Science

Nat. Rev. Cell. Biol.

Annu. Rev. Biochem.

Science

Nat. Mol. Cell. Biol.

Nat. Methods

Sensors

Nat. Biotechnol.

J. Am. Chem. Soc.

. J. Am. Chem. Soc.

J. Am. Chem. Soc.

Chem. Biol. Drug Des.

Science

Mol. BioSyst.