Turn-on fluorescent probe with visible light excitation for labeling of hexahistidine tagged protein
Graphical abstract
A novel fluorescein-based probe is reported. This molecule shows selective fluorescence enhancement on binding to a hexahistidine tag on the protein surface.
Section snippets
Acknowledgments
This work was supported in part by a Grant-in-Aid for University and Society Collaboration (12793009) to T.H., and a Grant-in-Aid for Young Scientists (B) (19790094) to N.U. from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, and by a Grant-in-Aid for Research in Nagoya City University to N.U. We thank Professor H. Sajiki and Dr. Y. Monguchi (Gifu Pharmaceutical University) for HRMS-FAB analysis.
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