ScienceDirect - Biosensors and Bioelectronics : Poly-(3-hexylthiophene) self-assembled monolayer based cholesterol biosensor using surface plasmon resonance technique

Biosensors and Bioelectronics
Volume 22, Issue 11, 15 May 2007, Pages 2516-2524

Font Size:

Abstract - selected

Article Toolbox

E-mail Article

Add to my Quick Links

Bookmark and share in 2collab (opens in new window)

Request permission to reuse this article

Cited By in Scopus (8)

Related Articles in ScienceDirect

Application of octadecanethiol self-assembled monolayer...
Talanta

Recent advances in cholesterol biosensor
Biosensors and Bioelectronics

Cholesterol biosensor based on N-(2-aminoethyl)-3-amino...
Analytical Biochemistry

Amperometric cholesterol biosensors based on the electr...
Talanta

Development of disposable lipid biosensor for the deter...
Biosensors and Bioelectronics

View More Related Articles

View Record in Scopus

doi:10.1016/j.bios.2006.10.011

Poly-(3-hexylthiophene) self-assembled monolayer based cholesterol biosensor using surface plasmon resonance technique

References and further reading may be available for this article. To view references and further reading you must purchase this article.

Sunil K. Arya^a^,^c, Pratima R. Solanki^a, S.P. Singh^a, K. Kaneto^b, Manoj K. Pandey^a, Monika Datta^c and Bansi D. Malhotra^a^,^,

^aBiomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India

^bDepartment of Biological Functions and Engineering, Kyushu Institute of Technology, Graduate School of Life Sciences and Systems Engineering, 3-4, Hibinko, Wakamitsu-ku, Kitakyushu 808-0196, Japan

^cDepartment of Chemistry, University of Delhi, Delhi 110007, India

Received 3 May 2006;

revised 29 September 2006;

accepted 3 October 2006.

Available online 17 November 2006.

Abstract

Cholesterol oxidase (ChOx) has been covalently immobilized onto 1-fluoro-2-nitro-4-azidobenzene (FNAB) modified poly-(3-hexylthiophene) (P3HT) self-assembled monolayer (SAM) onto gold coated glass plates. These ChOx/FNAB/P3HT/Au bio-electrodes have been characterized using contact angle (CA) measurements, UV–vis spectroscopy, electrochemical impedance technique, cyclic voltammetric technique and atomic force microscopic (AFM) technique, respectively. The ChOx/FNAB/P3HT/Au bio-electrodes were utilized for the estimation of cholesterol concentration in standard solutions using surface plasmon resonance (SPR) technique. It is shown that this SPR biosensor has linearity from 50 to 500 mg/dl of cholesterol in solution with detection limit of 50 mg/dl, sensitivity of 1.04 m°/(mg dl), reusability of around 15 times and a shelf-life of about 10 weeks when stored at 4 °C.

Keywords: Cholesterol; Cholesterol oxidase (ChOx); Surface plasmon resonance (SPR); Poly-(3-hexylthiophene) (P3HT); Electrochemical impedance; 1-Fluoro-2-nitro-4-azidobenzene (FNAB)