Elsevier

Aquaculture

Volume 321, Issues 3–4, 1 December 2011, Pages 237-244
Aquaculture

Very early weaning of common sole (Solea solea L.) larvae by means of different feeding regimes and three commercial microdiets: Influence on performances, metamorphosis development and tank hygiene

https://doi.org/10.1016/j.aquaculture.2011.09.007Get rights and content

Abstract

We studied the feasibility of weaning common sole (Solea solea L.) larvae at 13 days post hatching (dph) using commercial microdiets (MDs) while reducing live-feed utilization. Four dph larvae were fed two kinds of experimental weaning feeding regimens (FRs): control FR-C (AgloNorse, Ewos) where larvae were weaned onto MD at 27 dph; whilst the other FRs provided live feed until 13 dph, employing AgloNorse, Ewos (FR-A), Gemma, Skretting (FR-G) and O.range Large, INVE (FR-O). The trial lasted 29 days. No statistical differences among groups were observed in survival rates at the end of the trial. FR-A, FR-G and FR-O showed significantly lower weight and length increase when compared to control, whereas metamorphosis development was not influenced. Fatty acids larvae profile was significantly influenced by FR. The total number of Vibrio spp. was significantly higher in FR-C tanks at 21 dph. Salinity stress test values were not influenced by treatments. In conclusion, a very early weaning at 13 dph using commercial MDs in common sole larvae affects growth performance but not survival rate and metamorphosis development. A shorter live feed utilization improved tank hygiene.

Highlights

► Common sole larvae were weaned at 13 days post hatching using three commercial diets. ► Survival and metamorphosis were not affected by early weaning. ► Early weaned larvae showed lower growth performance. ► Early weaning reduced Vibrio spp. load in water.

Introduction

Common sole (Solea solea L.) is a very promising candidate for European aquaculture, characterized by high flesh quality, high market value and a total production of ~ 30 tons per year (Howell et al., 2008). There are though, several critical factors to be overcome in order to standardize a massive farming production: feeding behavior, susceptibility to disease, stocking density (Day et al., 1997, Howell, 1997, Imsland et al., 2004, Schram et al., 2006), as well as juvenile production (Howell et al., 2008) especially around weaning and metamorphosis which are accompanied by increased mortality rates (Rueda-Jasso et al., 2005). Standard feeding regimens during these periods represent a bottleneck for fishfarmers due to the required administration to young larvae of live-feed usually characterized by: 1) variable availability and price fluctuations of Artemia cysts (Callan et al., 2003), which can reach 700% (Moretti et al., 2005); and 2) poor hygienic conditions and high levels of pathogenic bacteria (Olafsen, 2001, Olsen et al., 2000). Total replacement of live-feed is still far to happen but the possibility of an early weaning of larvae using microdiets (MDs) could represent a valuable strategy to improve performances and reduce live feed utilization. First-time feeding fish larvae typically have a rudimentary digestive system characterized by a non-functional stomach and low digestive enzymes activity which make it difficult for them to process and assimilate a complex MD (Kolkovski, 2001). During the last decades, the pre-weaning period has been greatly reduced due to conclusive results obtained in laboratory (Cahu and Zambonino Infante, 2001). Furthermore, the utilization of a co-feeding regimen, which gradually weans larvae off live preys has been able to promote digestive maturation at early age (Engrola et al., 2007, Engrola et al., 2009) and to improve growth performances and survival rate of marine fish larvae (Rosenlund et al., 1997). In order to cover the knowledge gap on the use of commercial dry MDs for common sole before metamorphosis, we tested three different commercial products for their effects on growth performances, metamorphosis development and tank hygiene when used for an early weaning feeding regimen (FR).

Section snippets

Experimental feeding regimes

Four experimental weaning FRs were used in triplicate (Fig. 1). Control (FR-C) was formulated according to the protocol used at Solea BV, IJmuiden, The Netherlands, which is one of the most important hatcheries for this species (Andries Kamstra, personal communication). This protocol established live feed (Artemia nauplii until 9 dph and subsequently enriched metanauplii) with dry feed as co-feeding until 27 dph. The MD used in FR-C was AgloNorse, Ewos, Norway. The other three FRs were

Results

At the end of the trial, larvae successfully completed metamorphosis and the percentage of fish with abnormal pigmentation was less than 1‰ in all the experimental groups. Survival rates, as shown in Table 2, ranged from 36.7% to 55.8% without any statistical difference between groups. SGR data (Table 2) did not show any statistical difference until 14 dph, before the beginning of different feeding regimens. From 14 dph to 25 dph, significant differences appeared between groups, the control

Discussion

The feasibility of early weaning in common sole is a key-factor in the industrialization of farming processes of this promising aquaculture species. According to our data on survival rate, pigmentation and metamorphosis degree it seems feasible to promote a very early weaning using commercial MDs, in place of more traditional feeding regimens characterized by live-feed. The lack of significant differences found in the present study between early weaned groups and control could indicate a

Acknowledgments

We thank Davide Aluisio, Gloria Matassoni and Silvia Testi for technical assistance, Elettra Pignotti for statistical analyses.

References (57)

  • B.R. Howell

    A re-appraisal of the potential of the sole, Solea solea (L.), for commercial cultivation

    Aquaculture

    (1997)
  • S. Kolkovski

    Digestive enzymes in fish larvae and juveniles implications and applications to formulated diets

    Aquaculture

    (2001)
  • W. Koven et al.

    Advances in the development of microdiets for gilthead seabream, Sparus aurata: a review

    Aquaculture

    (2001)
  • A. Kvåle et al.

    Leaching properties of three different micropaticulate diets and preference of the diets in cod (Gadus morhua L.) larvae

    Aquaculture

    (2006)
  • I. Lund et al.

    Effect of dietary arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid on survival, growth and pigmentation in larvae of common sole (Solea solea L.)

    Aquaculture

    (2007)
  • I. Lund et al.

    The influence of dietary concentrations of arachidonic acid and eicosapentaenoic acid at various stages of larval ontogeny on eye migration, pigmentation and prostaglandin content of common sole larvae (Solea solea L.)

    Aquaculture

    (2008)
  • P. Makridis et al.

    Control of the bacterial flora of Brachionus plicatilis and Artemia franciscana by incubation in bacterial suspensions

    Aquaculture

    (2000)
  • V.C. Nhu et al.

    Effect of early co-feeding and different weaning diets on the performance of cobia (Rachycentron canadum) larvae and juveniles

    Aquaculture

    (2010)
  • J.A. Olafsen

    Interactions between fish larvae and bacteria in marine aquaculture

    Aquaculture

    (2001)
  • A.I. Olsen et al.

    Effects of short term feeding of microalgae on the bacterial flora associated with juvenile Artemia franciscana

    Aquaculture

    (2000)
  • R. Palazzi et al.

    Larval and juvenile rearing of common sole (Solea solea L.) in the Northern Adriatic (Italy)

    Aquaculture

    (2006)
  • L. Ribeiro et al.

    Histological and histochemical development of the digestive system of Solea senegalensis (Kaup, 1858) larvae

    Aquaculture

    (1999)
  • A.J. Ritar et al.

    Nutritional and bacterial profiles of juvenile Artemia fed different enrichments and during starvation

    Aquaculture

    (2004)
  • G. Rosenlund et al.

    Co-feeding marine fish larvae with inert and live diets

    Aquaculture

    (1997)
  • E. Schram et al.

    Stocking density-dependent growth of Dover sole (Solea solea)

    Aquaculture

    (2006)
  • M. Villalta et al.

    Arachidonic acid enriched live prey induces albinism in Senegal sole (Solea senegalensis) larvae

    Aquaculture

    (2005)
  • M. Villalta et al.

    The effect of graded concentrations of dietary DHA on growth, survival and tissue fatty acid profile of Senegal sole (Solea senegalensis) larvae during the Artemia feeding period

    Aquaculture

    (2005)
  • AOAC Method 920.153

    Official Methods of Analysis of AOAC International

    (2000)
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