Correlation of Circulating miR-765, miR-93-5p, and miR-433-3p to Obstructive Coronary Heart Disease Evaluated by Cardiac Computed Tomography
Section snippets
Methods
The study was approved by institutional ethics committee (IRCCS Fondazione SDN, protocol no. 7-13). All recruited individuals provided informed consent, and the study protocol conformed to the ethical guidelines of the Declaration of Helsinki. During a period of 36 months, 250 consecutive patients were enrolled. Patients with known history of cancer, active infections, chronic, or immune-mediated diseases, were excluded from the study. Furthermore, subjects with cardiomyopathy, known CHD,
Results
The baseline characteristics of subjects enrolled are summarized in Table 1. Subgroup analysis comparing dyslipidemia treated versus untreated patients and male versus female gender showed no significant differences for the expression of the selected circulating microRNAs.
We prospectively investigated by qRT-PCR the circulating expression levels of miRNAs in a group of CHD patients (n = 66) compared with a group of HS (n = 29) undergoing to CCT (Figure 1). Molecular analysis showed a
Discussion
The present study established that high levels of circulating miRNAs were able to discriminate patients with CHD (let-7c-5p, miR-765, miR-483-5p, miR-31-5p, and miR-206) and, in particular, with obstructive CHD (miR-765, miR-93-5p, and miR-433-3p). Furthermore, pretest probability risk scores (Framingham and Morise scores) widely used for patient stratification were not able to detect CHD and discriminate obstructive CHD while the addition of circulating miRNA quantification provided
Conflict of interest
The authors have no conflicts of interest to declare.
Acknowledgments
T.I. is a postdoc with a specific project “Nuove tecniche in immunologia clinica” granted by “Programma VALERE” of the University of Campania “Luigi Vanvitelli”, Department of Advanced Clinical and Surgical Sciences. The abstract of this article was accepted as poster presentation at ESC congress 2019.
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Funding: This study was supported by Italian Ministry of Health grants: “Giovani Ricercatori 2011-12” (project code GR-2011-02349436) (T.I.) and “Ricerca Corrente 2018” (C.N).
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Both TI and EF are co-first authors and contributed equally to this manuscript.