ResearchBasic science: ObstetricsGestational diabetes mellitus alters maternal and neonatal circulating endothelial progenitor cell subsets
Section snippets
Materials and Methods
Women with GDM and healthy pregnant control subjects were recruited through obstetric clinics and advertisements. Women were studied from 24-32 weeks' gestation according to the obstetricians' best estimate of gestation, 1-2 postpartum days, and 6-8 postpartum weeks. Newborn infants were studied at 1-2 days of age. GDM was defined according to the American College of Obstetrics and Gynecology guidelines. Exclusion criteria included type 1 or 2 diabetes mellitus; illnesses known to affect
Results
Forty-six pregnant women were enrolled; 27 women with GDM and 19 control subjects with a normal GDM screening test. Control subjects had no history of GDM and were not taking any prescription medications. One control subject had a diagnosis of hypertriglyceridemia and smoked tobacco. Of the GDM group, 2 women had GDM with a previous pregnancy; 1 woman smoked tobacco, and 1 woman was on sertraline for depression. Age, parity, blood pressure, and prepregnancy BMI, based on self-reported weight,
Comment
Here, we demonstrate that CPCs are reduced in women with GDM, compared with pregnant control subjects, during the third trimester and early postpartum period. Additionally, in uncomplicated pregnancies, CPCs are higher in the third trimester, compared with 1-2 days and 6-8 weeks after delivery. Interestingly, CPCs in control subjects at 1-2 days after delivery approach levels that have been detected in nonpregnant individuals,13, 14 which suggests a quick return to baseline after delivery. Our
Acknowledgments
We thank research nurses Lucy Miller and Tammy Garrett and obstetric diabetes dietitian Barbara Amerman for assisting with protocol implementation; Dr Sandra Hoesli (Vanderbilt University) for assistance with laser Doppler imaging, and the Angiogenesis and Endothelial Progenitor Cell Core, which is run by Dr Jamie Case with analyses conducted by Myka Estes and Julie Mund.
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Supported by US Public Health Services Grants R21 HL08885 (D.A.I., L.S.H.), R01 HL094725 (D.A.I., L.S.H.), P30 CA82709 (D.A.I., L.S.H.), UL1 RR025761 (C.K.S., L.A.D., D.A.I), U10HD063094 (D.M.H., L.S.H.), and the Riley Children's Foundation (D.A.I., L.S.H.).
Cite this article as: Acosta JC, Haas DM, Saha CK, et al. Gestational diabetes mellitus alters maternal and neonatal circulating endothelial progenitor cell subsets. Am J Obstet Gynecol 2011;204:••••.