Original investigationTargeted Molecular Imaging of Antigen OC183B2 in Ovarian Cancers Using MR Molecular Probes
Section snippets
Cell Culture
The human ovarian cancer SKOV-3 cell line was obtained from American Type Culture Collection and maintained in the logarithmic phase at 37°C in 5% CO2 humidified air in RPMI-1640 medium supplemented with 10% bovine fetal serum, 2 mmol/L glutamine, penicillin (100 U/mL), and streptomycin (100 U/mL). The medium was changed every 2 to 3 days. All experiments were conducted in compliance with regulations of the Animal Welfare Committee of Peking University People’s Hospital.
Immunocytochemistry
Expression of OC183B2 in
Cell Culture
SKOV-3 cells were successfully cultured and harvested at their logarithmic phase during all the experiments. The cells took on a polygon shape, displayed higher nucleus/plasma ratios and good refraction, and showed an anchorage-dependent growth pattern under invert-phase contrast microscopy.
Expression of the Antigen OC183B2 in SKOV-3 Cells
Expression of the antigen OC183B2 in SKOV-3 cells was detected by immunocytochemistry analysis. The immunocytochemistry images revealed that OC183B2 was expressed in the cytoplasm and on the plasma membranes
Discussion
Extensive research aimed at developing efficient therapeutic strategies for ovarian carcinoma has not improved the mortality of ovarian carcinoma. Consequently, efforts are now focused on the early detection of ovarian carcinoma (18). Because of its capability to image some events at the cellular and subcellular levels, molecular MR imaging may offer the potential in the early detection of ovarian cancer. Our study has provided convincing evidence using in vivo mice models with regard to this
Conclusions
OCMab183B2 coupled to USPIO conjugates has demonstrated the potential to be useful as an OC183B2-targeted MR imaging contrast agent for monitoring ovarian cancers. Also, this work may provide a novel view for early detecting and differentiating tumors clinically.
Acknowledgments
We express our sincere appreciation to Professor Mingyuan Gao and his team at the Key Laboratory of Colloid, Interface Science and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, for preparations of PEG-coated nanoparticles and OCMab183B2 USPIOs MR probes.
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This work was funded by grant 7092111 from the Beijing Nature Science Foundation (Beijing, China).