Elsevier

Analytical Biochemistry

Volume 605, 15 September 2020, 113812
Analytical Biochemistry

Technical note
Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification

https://doi.org/10.1016/j.ab.2020.113812Get rights and content

Highlights

  • •

    Easy detection of cnm gene related to cerebral hemorrhage.

  • •

    Non-invasive sampling using saliva.

  • •

    Reduced detection time due to shorter culture period and not extracting DNA.

Abstract

This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in saliva without DNA extraction after 1 day of culture. This method resulted in a cnm-positive rate of 26.4% in 102 samples, which was higher than that obtained with conventional PCR. In conclusion, LAMP may be used for the detection of cnm-positive S. mutans in a large number of samples.

Keywords

cnm gene
Streptococcus mutans
Loop-mediated isothermal amplification

Cited by (0)

View Abstract
© 2020 Elsevier Inc. All rights reserved.