Notes & tipsProlonged treatment with 3-isobutyl-1-methylxanthine improves the efficiency of differentiating 3T3-L1 cells into adipocytes
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Acknowledgments
This work was funded by grants from the State Education Ministry of China (20130076120017) and the National Natural Science Foundation of China (31570382 and 31301191).
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2019, Analytical BiochemistryCitation Excerpt :In contrast, the differentiation efficiencies of 3T3-L1 cells induced by cocktails I3 and I6 were under 50%. These results were similar to the study reported by Hua et al., who failed to efficiently differentiate 3T3-L1 cells to adipocytes using a cocktail with 0.5 mM IBMX, 1 μM DEX, and 1 μg/mL insulin [10]. Furthermore, cell morphology during differentiation was altered.
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