Notes & TipsDetection of mutations in the β-globin gene by colorimetric staining of DNA microarrays visualized by a flatbed scanner
Section snippets
Acknowledgments
This work was supported by the Danish Research Council (grant 2014-00-0003, DABIC) and the AAse and Ejnar Danielssens Foundation.
References (11)
- et al.
Evaluation of thin films of agarose on glass for hybridization of DNA to identify plant pathogens with microarray technology
Anal. Biochem.
(2005) - et al.
Detection of mutations using microarrays of poly(C)10–poly(T)10 modified DNA probes immobilized on agarose films
Anal. Biochem.
(2006) - et al.
Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection
Genomics
(1998) - et al.
Catalyzed reporter deposition, a novel method of signal amplification: II. Application to membrane immunoassays
J. Immunol. Methods
(1991) - et al.
Colorimetric silver detection of DNA microarrays
Anal. Biochem.
(2001)
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These two authors contributed equally to this work.