Spectrophotometry | Overview

doi:10.1016/B978-0-12-409547-2.14265-9

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

Encyclopedia of Analytical Science (Third Edition)

2019, Pages 244-248

https://doi.org/10.1016/B978-0-12-409547-2.14265-9 Get rights and content

Abstract

Ultraviolet-visible (UV-vis) spectrophotometry, a widely used technique for quantitative analysis, relies on attenuation of a radiation beam by the analyte in solution. Near-ultraviolet (180–390 nm) or visible (390–780 nm) radiation is exploited to promote electronic transitions in a molecule. Some organic and inorganic species inherently absorb radiation in the UV-vis range, and the technique can be extended to non-absorbing analytes by exploiting a selective reaction of the analyte with an appropriate reagent to form an absorbing chemical species. This article provides an overview of the technique and includes sections on spectrum-structure relationships, quantitative aspects (based on Beer’s law), instrument design and applications.

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Citation Excerpt :
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Spectrophotometry | Overview☆

Abstract