Document heading
Prevalence and antibiogram of bacterial isolates from urinary tract infections at Dessie Health Research Laboratory, Ethiopia

https://doi.org/10.1016/S2221-1691(14)60226-4Get rights and content
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ABSTRACT

Objective

To determine the prevalence and antimicrobial susceptibility of bacteria from suspected urinary tract infections.

Methods

A retrospective analysis of bacterial pathogens and their antimicrobial susceptibility was done on urine samples at Dessie Regional Laboratory in the period 2003 to 2010. Antimicrobial susceptibility tests were done using disc diffusion technique as per the standard of Kirby-Bauer method.

Results

The male to female ratio of the patients was 1:1.96. Of the total 1404 samples, 319 (22.7%) were culture positive. Escherichia coli was the dominant isolate (63.6%) followed by Klebsiella spp. (8.5%) and Proteus spp. (8.2%). The overall resistance rates to erythromycin, amoxycillin, and tetracycline were 85.6%, 88.9% and 76.7%, respectively. The three most frequently isolated bacteria had resistance rates of 80.1%-90.0% to, amoxycillin, and tetracycline and sensitivity rates of 0 to 25% to nitrofurantoin, ciprofloxacin and gentamicin. Antibiogram of isolates showed that 152 (47.85%) isolates were resistance to two and more antimicrobials.

Conclusions

In the study area resistance rates to erythromycin, amoxycillin and tetracycline were high. Since most isolates were sensitive to nitrofurantoin and gentamicin, they are considered as appropriate antimicrobials for empirical treatment urinary tract infections.

KEYWORDS

Antimicrobial resistance bacteria
Ethiopia
Uropathogens

Cited by (0)

Financial Support: Supported by Bahir Dar University, Science College (Grant number SCPGRCS 002/2004).

Peer reviewer

Dr. T Arun, M.Sc, Ph.D, Assistant Professor, Department of Biology, Bahir Dar University, P.O. Box–79, Bahir Dar, Ethiopia.

Tel: +251 932816276

E-mail: [email protected]

Comments

The area of data collection by the authors is very much useful. The urine sample ratio is more in female than male. The highest isolation rate of uropathogens was obtained in the age group between 24 to 44 years. The antibiotic resistant pattern was observed only by Kirby-Bauer disc diffusion method.

Details on Page 167

Available online 28 Feb 2014