Elsevier

SLAS Technology

Volume 9, Issue 1, February 2004, Pages 16-23
SLAS Technology

Technical Report
Automating Procedures for Processing, Cryopreservation, Storage, and Manipulation of Human Peripheral Blood Mononuclear Cells

https://doi.org/10.1016/S1535-5535(03)00202-8Get rights and content
Under a Creative Commons license
open access

Cell-mediated immune responses have been found to play a crucial role in the protection from, or amelioration of, many human diseases. There are many vaccine trials being conducted where the most important immune assessment consists of measuring T cell responses. The most readily accessible source of T cells is the peripheral blood. Thus, collection, processing, cryopreservation, storage, and manipulation of human peripheral blood mononuclear cells (PBMCs) are all key steps for assessment of vaccine and disease-induced immune responses. Automation of these procedures is not currently practiced widely. This article reviews current practices and explores the need for automation.

Keywords

cellular immunity
cryopreservation
vaccine
human clinical trials
automation
blood processing

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