ArticleComparison of effects of zona drilling by non-contact infrared laser or acid Tyrode's on the development of human biopsied embryos as revealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics
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Having completed her preliminary education in Greece with distinction, Dr Chatzimeletiou was awarded a BSc (Hons) Degree in Biological Sciences from the University of Essex, an MSc with distinction in Human Reproductive Biology from Imperial College School of Medicine at Hammersmith Hospital in London, and a PhD in Preimplantation Genetics and Embryology from the University of Leeds. She was appointed as a Research Assistant in the Department of Obstetrics and Gynaecology at St Thomas' Hospital
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The human embryo following biopsy on day 5 versus day 3: viability, ultrastructure and spindle/chromosome configurations
2022, Reproductive BioMedicine OnlineCitation Excerpt :A negative control using non-immune serum (1:20 dilution, Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA) was also included (Supplementary Figure 1). All the blastocysts were analysed using the Zeiss Imager.Z1 fluorescence microscope and images were captured using the ISIS software (Metasystems, Antisel, Thessaloniki, Greece) and/or a Leica TCS-SP laser scanning confocal microscope (Analytical Instruments SA, Athens, Greece), with confocal image analysis typically accomplished by capturing a z-series stack of 1 µm thick sections encompassing the entire blastocysts (Chatzimeletiou et al., 2005a,b, 2012, 2019). Classification of abnormalities in SCC were as described in Chatzimeletiou et al. (2005a,b, 2012).
Viability assessment using fluorescent markers and ultrastructure of human biopsied embryos vitrified in open and closed systems
2021, Reproductive BioMedicine OnlineCitation Excerpt :Following warming according to the manufacturer's instructions, the blastocysts were allowed to recover in culture for 4 h before being subjected to viability staining with CFSE/PI or processed for TEM. Blastocyst viability after vitrification–warming was assessed using two fluorescent markers as previously described (Chatzimeletiou et al., 2001, 2005a; Oikonomou et al., 2017): (i) carboxyfluorescein-diacetate succinimidylester (CFSE; Molecular Probes Inc., Cambridge Bioscience, Cambridge, UK), which binds covalently in the cytoplasm of viable cells and stains them green and (ii) propidium iodide (PI) (Sigma, Dorset, UK), which enters cells with damaged membranes, binding to double-stranded DNA in the nucleus, labelling it red while it is excluded from viable cells. Fresh and vitrified–warmed blastocysts were first incubated in CFSE (20 µg/ml) in Ca2+/Mg2+-free PBS (Gibco; Thermo Fisher Scientific, Logan, Utah, U.S.A.) for 30 min at 37°C and then in PI (10 µg/ml).
Preimplantation genetic testing: a review of current modalities
2021, F and S ReviewsCitation Excerpt :Although not extensively studied, available data show no significant difference in embryo outcome with acidic treatment versus laser treatment of the ZP for PGT. However, use of acidic digestion did require significantly more time to complete compared with use of the laser (21–23). A single cell contains ∼6 pg DNA, which is not adequate for testing.
Laser technology in the ART laboratory: a narrative review
2019, Reproductive BioMedicine OnlineCitation Excerpt :Moreover, laser equipment enables multiple embryos to be kept in the same dish as they do not need to be washed from the acid afterwards. Studies comparing the use of acid Tyrode's with laser zona drilling for embryo biopsy have shown that laser drilling is an easier and faster procedure, resulting in a greater number of intact blastomere cells, minimizing the time the embryo spends outside the incubator, and often leading to increased pregnancy success rates (Chatzimeletiou et al., 2005; Geber et al., 2011; Joris et al., 2003). Before laser-assisted procedures, the biopsy technique was limited by both operator skill and a non-standardized tunnel size.
Micromanipulation in assisted reproductive technology
2016, Reproductive BioMedicine OnlineTechnology requirements for preimplantation genetic diagnosis to improve assisted reproduction outcomes
2010, Fertility and Sterility
Having completed her preliminary education in Greece with distinction, Dr Chatzimeletiou was awarded a BSc (Hons) Degree in Biological Sciences from the University of Essex, an MSc with distinction in Human Reproductive Biology from Imperial College School of Medicine at Hammersmith Hospital in London, and a PhD in Preimplantation Genetics and Embryology from the University of Leeds. She was appointed as a Research Assistant in the Department of Obstetrics and Gynaecology at St Thomas' Hospital in London and she is currently the molecular cytogenetic laboratory manager, responsible for the preimplantation genetic screening programme, at the London Bridge Fertility Gynaecology and Genetics Centre. Her research interests include chromosomal and cytoskeletal abnormalities in human embryos, stem cells, prenatal and preimplantation genetic diagnosis.