ReviewThe aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa
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Bacterial strains and growth conditions
P. aeruginosa PAO1 (15) and its derivatives were used in this study. E. coli MV1184 (16) and HB101 (17), which were used for plasmid construction and DNA manipulation, were grown at 37°C with shaking in 2×YT medium (18) supplemented with appropriate antibiotics. This medium was also used for the P. aeruginosa cells for aerotaxis and electroporation.
Aerotaxis assay
The chemotaxis well chamber method (9) was used to assess aerotaxis. P. aeruginosa strains were transformed with plasmid pMRP9-1 (19), which
Aerotaxis is induced upon entry into the stationary phase
Aerotactic responses of P. aeruginosa were assessed with the chamotaxis well chamber method (9). When both of the upper and lower wells contained HEPES buffer alone, P. aeruginosa (pMRP9-1) moved from the lower to the upper well through the filter, responding to the gradient of oxygen. After the gfp-tagged P. aeruginosa PAO1 cells were introduced to the lower well, the GFP fluorescence intensity in the upper well continuously increased (Fig. 1).
Aerotactic responses of cells were quantitated
Discussion
P. aeruginosa is able to grow under anaerobic conditions using nitrate as a terminal electron acceptor for anaerobic respiration (33). The arginine deiminase pathway enables P. aeruginosa to generate ATP under anaerobic conditions in the absence of nitrate using arginine as a fermentation substrate (34). The denitrification and arginine deiminase pathways are repressed under aerobic conditions (35, 36). This means that this bacterium prefers an aerobic respiratory metabolism to anaerobic
References (50)
- et al.
Chemotaxis away from thiocyanic and isothiocyanic esters in Pseudomonas aeruginosa
FEMS Microbiol. Lett.
(1993) - et al.
Evaluation of bacterial aerotaxis for its potential use in detecting the toxicity of chemicals to microorganisms
J. Biotechnol.
(2003) - et al.
Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa
FEMS Microbiol. Lett.
(2004) - et al.
Production of single-stranded plasmid DNA
Methods Enzymol.
(1987) - et al.
Quorum sensing signals in development of Pseudomonas aeruginosa biofilms
Methods Enzymol.
(1999) - et al.
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
Gene
(1989) - et al.
Self perception in bacteria: quorum sensing with acylated homoserine lactones
Curr. Opin. Microbiol.
(1998) - et al.
Quorum sensing as a population-density-dependent determinant of bacterial physiology
Adv. Microb. Physiol.
(2001) - et al.
Quorum-sensing in gram-negative bacteria
FEMS Microbiol. Rev.
(2001) - et al.
The structural genes for nitric oxide reductase from Pseudomonas aeruginosa
Biochim. Biophys. Acta
(1995)
Chemotaxis in bacteria
Science
Bacterial chemotaxis and the molecular logic of intracellular signal transduction networks
Annu. Rev. Biophys. Biophys. Chem.
Molecular cloning and characterization of a chemotactic transducer gene in Pseudomonas aeruginosa
J. Bacteriol.
Regulation of Pseudomonas aeruginosa chemotaxis by the nitrogen source
J. Bacteriol.
Chemotaxis by Pseudomonas aeruginosa
J. Bacteriol.
Chemotaxis in Pseudomonas aeruginosa
J. Bacteriol.
Rapid method for analyzing bacterial behavioral responses to chemical stimuli
Appl. Environ. Microbiol.
Phosphate taxis in Pseudomonas aeruginosa
J. Bacteriol.
The Aer protein and the serine chemoreceptor Tsr independently sense intracellular energy levels and transduce oxygen, redox, and energy signals for Escherichia coli behavior
Proc. Natl. Acad. Sci. USA
A signal transducer for aerotaxis in Escherichia coli
J. Bacteriol.
Identification of two chemotactic transducers for inorganic phosphate in Pseudomonas aeruginosa
J. Bacteriol.
Chromosomal genetics of Pseudomonas
Microbiol. Rev.
A complementation analysis of the restriction and modification of DNA in Escherichia coli
J. Mol. Biol.
Molecular cloning: a laboratory manual
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