Journal of Pharmacological and Toxicological Methods
Original articleEfficient fixation procedure of human leukemia cells in sulforhodamine B cytotoxicity assay☆
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2020, Bioorganic ChemistryCitation Excerpt :Vorinostat was used as positive control. After 72 h of cultivation, the plates were assayed for cell cytotoxicity/viability by using the following two methods: Sulforhodamine B (SRB) method [13,34,35] and CellTiter-Glo® Luminescent Cell Viability Assay or CTG method (Promega Corporation, Madison, WI, USA). The raw data were processed using BioTek’s Software Gen5 (v2.03.01) to generate inhibitory IC50 values.” [13].
Fed-batch production of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in soluble form in Escherichia coli and its purification and characterization
2016, Protein Expression and PurificationCitation Excerpt :The hollow cathode lamp of zinc (10-mA lamp current), 213.8 nm wave length, air-acetylene flame, and 1.3 nm slit width were selected in this experiment. Cytotoxicity of TRAIL was determined with a sulforhodamine B assay [19]. BEL-7402 human hepatocellular carcinoma cells (from Shanghai Institute of Material Medical, Chinese Academy of Sciences) were seeded in 96-well plates (1 × 104/well) for 24 h, then removed the supernatant and added serially diluted TRAIL.
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This work was supported by HAN project of Ministry of Science and Technology, Korea.