Unravelling the Leishmania genome

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Abstract

The past few years have seen significant advances in our understanding of eukaryotic genomes. In the field of parasitology, this is best exemplified by the application of genome mapping techniques to the study of genome structure and function in the protozoan parasite, Leishmania. Although much is known about the organism and the diseases it causes, molecular genetics has only recently begun to play a major part in elucidating some of the unusual characteristics of this interesting parasite. Mapping of the small (35 Mb) genome and determination of the functional role of genes by the application of in vitro homologous gene targeting techniques are revealing novel avenues for the development of prophylactic measures.

References (67)

  • Y Kohara et al.

    The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library

    Cell

    (1987)
  • A Coulson et al.

    Towards a physical map of the genome of the nematode Caenorhabditis elegans

    Proc Natl Acad Sci USA

    (1986)
  • AC Ivens et al.

    Cosmid clones and their application to genome studies

  • J Sulston et al.

    Software for genome mapping by fingerprinting techniques

    Comput Appl Biosci

    (1988)
  • GR Franco et al.

    Identification of new Schistosoma mansoni genes by the EST strategy using a directional cDNA library

    Gene

    (1995)
  • KL Wan et al.

    Toxoplasma gondii expressed sequence tags: insight into tachyzoite gene expression

    Mol Biochem Parasitol

    (1996)
  • MS Boguski et al.

    dbEST — database for expressed sequence tags

    Nat Genet

    (1993)
  • H Shau et al.

    Identification of natural killer enhancing factor as a major antioxidant in human red blood cells

    Biochem Biophys Res Commun

    (1994)
  • M Francis et al.

    Salmonellae may mount a coordinated stress response to free O2 radicals produced within macrophage phagosomes

    Infect Immun

    (1993)
  • CJ Soong et al.

    Protection of gerbils from amoebic liver abscess by immunisation with recombinant Entamoeba histolytica 29 kilodalton antigen

    Infect Immun

    (1995)
  • JA Rioux et al.

    Taxonomy of Leishmania, use of isoenzymes, suggestions for a new classification

    Ann Parasitol Hum Comp

    (1990)
  • MHL De Brujin et al.

    A comparative study of diagnosis by the polymerase chain reaction and by current clinical methods using biopsies from Colombian patients with suspected leishmaniasis

    Trop Med Parasitol

    (1993)
  • TW Spithill et al.

    The molecular karyotype of Leishmania major and mapping of α and β tubulin gene families to multiple unlinked chromosomal loci

    Nucleic Acids Res

    (1985)
  • JK Scholler et al.

    Molecular karyotype of species and subspecies of Leishmania

    Mol Biochem Parasitol

    (1986)
  • P Bastien et al.

    Molecular karyotype analysis in Leishmania

  • P Wincker et al.

    The Leishmania genome comprises 36 chromosomes conserved across widely divergent human pathogenic species

    Nucleic Acids Res

    (1996)
  • MP Levick et al.

    An expressed sequence tag analysis of full-length, spliced-leader cDNA libraries from Leishmania major promastigotes

    Mol Biochem Parasitol

    (1996)
  • S Foote et al.

    The human Y chromosome: overlapping DNA clones spanning the euchromatic region

    Science

    (1992)
  • I Chumakov et al.

    Continnum of overlapping clones spanning the entire human chromosome 21 q

    Nature

    (1992)
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