Minimal Requirements for Growth of Brucella suis and Other Brucella Species
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Brucella abortus RB51 ΔleuB expressing Salmonella FliC conjugated gonadotropins reduces mouse fetal numbers: A possible feral swine brucellosis immunocontraceptive vaccine
2021, HeliyonCitation Excerpt :This study reports a leucine auxotroph of B. abortus RB51 expressing gonadotropins conjugated to Salmonella typhimurium FliC reduces fertility in a murine model. This data, plus previously published data from our lab, justifies further testing of these vaccine candidates in feral swine [27, 28]. Brucella abortus RB51 ΔleuB (RB51L), B. abortus RB51 ΔleuB pNS4-TrcD-FliC-porcineFSHbeta (RB51LFSHβ), and B. abortus RB51 ΔleuB pNS4-TrcD-FliC-GnRH (RB51LGnRH) from the culture collection at Virginia-Maryland College of Veterinary Medicine (VMCVM) (Blacksburg, VA).
Erythritol triggers expression of virulence traits in Brucella melitensis
2013, Microbes and InfectionCitation Excerpt :All B. melitensis strains were maintained on brucella agar and grown in brucella broth (Becton Dickinson) at 37 °C. Microarray analysis and promoter activity levels of B. melitensis were conducted in yeast extract minimal medium (YEMM) [12]. E. coli was grown in Luria-Bertani broth at 37 °C.
Development and evaluation of a selective medium for Brucella suis
2012, Research in Veterinary ScienceOver-expression of homologous antigens in a leucine auxotroph of Brucella abortus strain RB51 protects mice against a virulent B. suis challenge
2011, VaccineCitation Excerpt :The B. abortus strain RB51leuB from our culture collection was made competent as described earlier [18,20]. Brucella minimal medium (BMM) was prepared as described by Plommet [21]; all incubations were done at 37 °C in the presence of 5% CO2. Standard laboratory procedures recommended by Centers for Disease Control and Prevention (USA) were followed while handling live Brucella in a Biosafety Level 3 facility of the Virginia-Maryland Regional College of Veterinary Medicine.
Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens
2009, Infection, Genetics and EvolutionBrucella evolution and taxonomy
2002, Veterinary Microbiology
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Dr. M. Plommet, Station de Pathologie de la Reproduction, INRA, F-37380 Nouzilly, France