Application of the charged polymeric materials as additional permselective membranes for improvement of the performance characteristics of urea-sensitive ENFETs 2. Urea determination in blood serum

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Abstract

An improved urea-sensitive biosensor based on pH-sensitive field-effect transistors was prepared by cross-linking urease with bovine serum albumin in a saturated glutaraldehyde vapour on the sensor chips. Additional Nafion or PVP (poly (4-vinylpyridine-co-styrene)) membranes were deposited on the top of enzymatic and reference membranes of urea biosensors by a spin-coating process. It was shown that the presence of such permselective additional layers removes the non-specific response coming from high concentration of proteins and other components in real biological samples and furthermore increases the biosensor stability. The use of enzymatic field-effect transistors coated with additional membranes allows analysis of urea in serum with increased sensitivity and accuracy.

Keywords

Biosensor
Urea sensitive ENFET
Polymeric permselective charged membranes
Additional membranes
Nafion
PVP
Urea detection in serum

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