Affinity characterization of monoclonal and recombinant antibodies for multianalyte detection with an optical transducer
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Real-time monitoring of immunoglobulin G levels in milk using an ordered porous layer interferometric optical sensor
2022, TalantaCitation Excerpt :SPR is a new nonlabelled biosensor technology that can also be used to detect protein activity, but it needs expensive gold films and complex instruments [16]. Reflectometric interference spectroscopy (RIfS), which can measure small changes in the optical thickness (OT) of a biomolecular layer on a surface, has increasingly emerged as a powerful analysis technology for the investigation of protein–protein interactions [18–21]. Interference substrates are the most important part of the RIfS platform.
Battery-triggered ultrasensitive electrochemiluminescence detection on microfluidic paper-based immunodevice based on dual-signal amplification strategy
2013, Analytica Chimica ActaCitation Excerpt :Similarly, Ab1 and BSA could all resist the electron-transfer kinetics of the redox probe at the fiber surface, resulting in the increased of resistance (curves c and d), which verified the immobilization of these substances. Cross-talk [33] and cross-reactivity [34] are two crucial factors regarding the specificity, and thus the reliability of MIA. In this investigation, according to the design of the electrochemical cell in the paper-based immunodevice (Scheme 1), the diffusion of signal reporter (P-acid/NPS-labeled Ab2) from one paper working zone to another should go through two long paper channels and one paper auxiliary zone, and the washing direction could effectively exclude this diffusion.
Cross-talk-free multiplexed immunoassay using a disposable electrochemiluminescent immunosensor array coupled with a non-array detector
2011, Biosensors and BioelectronicsCitation Excerpt :Therefore, a PBS (0.10 M, pH 7.0) containing 0.10 M TPA was adopted as the ECL co-reactant. Cross-talk (Wilson and Nie, 2006) and cross-reactivity (Piehler et al., 1997) are two crucial factors regarding the specificity, and thus the reliability of MIA. In this investigation, the ECL emitter was the excited Ru(bpy)32+, and it was bound onto the surface of the immunosensor via the immuno-binding.
Biosensor-based estimation of kinetic and equilibrium constants
2001, Analytical BiochemistryQuality Issues of Research Antibodies
2016, Analytical Chemistry InsightsComparison of an indirect format ELISA on modified graphite and polystyrene surfaces against triazines
2006, Zeitschrift fur Naturforschung - Section C Journal of Biosciences