Regular paper
Enhanced production of l(+)-lactic acid from corn starch in a culture of Rhizopus oryzae using an air-lift bioreactor

https://doi.org/10.1016/S0922-338X(97)82063-6Get rights and content

Abstract

l(+)-Lactic acid production was investigated using 8 Rhizopus strains and various culture conditions. Rhizopus oryzae NRRL395 showed the highest yield of l(+)-lactic acid among various Rhizopus strains. The optimum production medium contained the following: corn starch, 120 g/l; ammonium sulfate, 1.35 g/l; small amounts of mineral salts (potassium phosphate, magnesium sulfate and zinc sulfate). The data obtained for the flask culture were successfully reproduced using a 3 l air-lift bioreactor under the optimum conditions. The yield (based on initial carbon source concentration) and final concentration of l(+)-lactic acid were 85% and 102 g/l, respectively, in the 3 l air-lift bioreactor. The lactic acid was recovered with a yield (based on lactic acid containing in broth) of 90% using 4 steps, i.e., filtration, active carbon treatment, ultra-filtration and concentration by vacuum evaporation. The lactic acid purified was 100% l(+)-form as judged by HPLC.

References (10)

  • M. Okabe et al.

    Itaconic acid production in an air-lift bioreactor using a modified draft tube

    J. Ferment. Bioeng.

    (1993)
  • C. Soccol

    Physiologie et metabolisme de Rhizopus en culture solide et submergee en relation avec la degradation d'amidon cru et la production d'acide l(+) lactique

  • M. Vert et al.

    Des biosystemes aux materiaux polymeres: une utopie

    Biofutur

    (1992)
  • T.B.V. Roy et al.

    lactic acid production by Lactobacillus delbreuckii in a hollow fiber fermentor

    Biotechnol. Lett.

    (1982)
  • L.B. Lockwood

    Organic acid production of acids

There are more references available in the full text version of this article.

Cited by (118)

  • Effects of oxygen transfer rate on the L(+) lactic acid production by Rhizopus oryzae NRRL 395 in stirred tank bioreactor

    2022, Biochemical Engineering Journal
    Citation Excerpt :

    Unlike bacterial fermentation, it has been found that filamentous fungi of the genus Rhizopus can produce lactic acid at lower costs using agro-industrial residues or by-products, which are able to transform these complex materials into biomass and high value-added products [11–13]. The most studied fungus for LA production has been R. oryzae; in particular, the highest yields have been found with the strain R. oryzae NRRL 395 [14,15]. This microorganism possesses an enzymatic battery which enables it to utilize complex residues and produce only the L(+)-lactic acid isomer efficiently in a single step [13,16].

  • Attenuation of UV absorption by poly(lactic acid)-iron oxide nanocomposite particles and their potential application in sunscreens

    2021, Chemical Engineering Journal
    Citation Excerpt :

    PLA does not have significant ultraviolet absorption and has been used as a nano-encapsulation system to stabilize octyl methoxy cinnamate, an organic ultraviolet filter widely used in cosmetics and sunscreen [36]. In addition, the ‘eco-friendly’ properties of lactic acid-based materials are highly prospective for commercial development [37]. In this study, we seek to improve the efficacy of cosmetic products by using Fe2O3 nanoparticles incorporated into a PLA matrix.

  • Technological aspects of the production of biodegradable polymers and other chemicals from renewable sources using lactic acid

    2017, Journal of Cleaner Production
    Citation Excerpt :

    However, one disadvantage of lactic acid bacteria is the need for the use of expensive growth factors in the nutrient medium (Altaf et al., 2006; Hofvendahl and Hahn-Hugerdal, 2000; Van Niel and Hahn-Hugerdal, 1999). On the other hand, the use of fungal and yeast producers typically decreases the yield and final concentration of the lactic acid produced; in some cases, a part of the carbohydrate substrate is also converted into ethanol and unwanted organic acids, which complicate the subsequent stages of isolation and purification of lactic acid (Ishida et al., 2005; Sauer et al., 2010; Yin et al., 1997). Today, industrial biosynthesis of lactic acid is preferably carried out by periodic cultivation.

View all citing articles on Scopus
View full text