Genotypic and Phenotypic Diversity of Pseudomonas stutzeri

https://doi.org/10.1016/S0723-2020(11)80294-8Get rights and content

Summary

A total of 49 strains of Pseudomonas stutzeri including reference strains from culture collections were phenotypically and genotypically studied. They were isolated from clinical sources, marine sediments, waste-water treatment plants and soil. DNA-DNA hybridization using the thermal melting point of the hybrids as parameter established seven genomic groups, four of them containing less than three strains. The G+C content ranged from 60.9–64,9 mol%. Phenotypical investigation using 102 characters demonstrated a considerable heterogeneity within the genomic groups. Because of the lack of useful differential tests there is still no sound basis for division of P. stutzeri at the species level. It is proposed that the term ‘genomovar’ be used to denote genomic groups of a nomenspecies.

References (22)

  • B. Frantz et al.

    Degradative plasmids in Pseudomonas

  • J. Tamaoka et al.

    Determination of DNA base composition by reversed-phase high-performance liquid chromatography

    FEMS Microbiol. Lett.

    (1984)
  • P. Baumann et al.

    Taxonomy of Marine Pseudomonas species: P. stanieri sp. nov.; P. perfectomarina sp. nov., nom. rev.; P. nautica; and P. doudoroffi

    Int. J. System. Bact.

    (1983)
  • R. Burri et al.

    Ueber Nitrat zerstörende Bakterien und den durch dieselben bedingten Stickstoffverlust

    Zbl. Bakt., II. Abt.

    (1895)

    Zbl. Bakt., II. Abt.

    (1895)

    Zbl. Bakt., II. Abt.

    (1895)

    Zbl. Bakt., II. Abt.

    (1895)
  • S.T. Cowan

    Cowan and Steel's manual for the identification of medical bacteria

    (1974)
  • K.V. Döhler et al.

    Transfer of Pseudomonas perfectomarina Baumann, Bowditch, Baumann, and Beaman 1983 to Pseudomonas stutzeri (Lehmann and Newmann 1986) Sijderius 1946

    Int. J. System. Bact.

    (1987)
  • E. García-Valdés et al.

    New naphthalene-degrading Pseudomonas strains

    Appl. Environ. Microbiol

    (1988)
  • F. Gavini et al.

    Numerical taxonomy of Pseudomonas alcaligenes, P. pseudoalcaligenes, P. mendocina, P. stutzeri, and related bacteria

    Int. J. System. Bact.

    (1989)
  • B. Holmes

    Identification and distribution of Pseudomonas stutzeri in clinical material

    J. Appl. Bact.

    (1986)
  • E. Lind et al.

    Clinical strains of Enterobacter agglomerans (synonyms: Erwinia herbicola, Erwinia milletiae) identified by DNA-DNA hybridization

    Acta Path. Microbiol. Immunol. Scand. Sect. B

    (1986)
  • M. Mandel

    Deoxyribonucleic acid base composition in the genus Pseudomonas

    J. Gen. Microbiol.

    (1966)
  • Cited by (96)

    • Do plastics serve as a possible vector for the spread of antibiotic resistance? First insights from bacteria associated to a polystyrene piece from King George Island (Antarctica)

      2019, International Journal of Hygiene and Environmental Health
      Citation Excerpt :

      This strain was originally isolated from the water of a lagooning wastewater treatment plant by enrichment with 2-methylnaphthalene band and classified as a new denitrifying species of Pseudomonas (Bennasar-Figueras et al., 2016). Its physiological tolerance to 8.5% NaCl suggested that P. balearica represents a true marine Pseudomonas species; other strains of P. balearica were previously isolated from marine areas (Rossello et al., 1991) and salt marshes (Mulet et al., 2008). ANT-2B(1) strain was related to Thalassospira lohafexi, a gram-negative, aerobic, obligatory halophilic bacterium, which was isolated also from the Southern Ocean water samples surrounding Antarctica (Shivaji et al., 2015).

    • Diversity of bacteria in ships ballast water as revealed by next generation DNA sequencing

      2016, Marine Pollution Bulletin
      Citation Excerpt :

      Actinobacteria are also predominant in the bacterioplankton of marine habitats (8.2%; Biers et al., 2009) but contributed < 1% of ballast water samples from ships conducting BWEs. Bacteria from ship C (Table 2), that did not conduct a BWE because it contained coastwise ballast water, were predominantly Gammaproteobacteria (39.7%) with most within the Pseudomonadales (18.6%), typically found in freshwater and terrestrial soils (Molina et al., 2014; Peix, Ramirez-Bahena, and Velazquez, 2009; Rossello et al., 1991), which reflects the influence of the lower Mississippi River, the source of the ballast water, on the sample. Other Gammaproteobacteria included the Alteromonadales (6.4%), the Methylococcales (2.0%) (i.e. methylotrophs), the Legionellales (i.e. Legionella pathogens) (2.0%), the Oceanospirillales (2.4%) and the Vibrionales (2.4%) (i.e. Vibrio species including the human pathogen Vibrio vulnificus).

    View all citing articles on Scopus
    1

    R. Rosselló, Laboratorio de Microbiología, Departamento de Biología y Ciencias de la Salud, Facultad de Ciencias, Universidad de las Islas Baleares, Crtra Valldemossa Km 7.5, 07071 Palma de Mallorca, Spain

    View full text