Development of Diagnostic Oligonucleotide Probes for Four Lactobacillus Species Occurring in the Intestinal Tract
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2010, Journal of Environmental SciencesApplication of molecular identification tools for Lactobacillus, with a focus on discrimination between closely related species: A review
2009, LWTCitation Excerpt :Probes are synthetically prepared oligonucleotides meant to bind to the specific complimentary recognition sequence on the target bacterial genomic DNA. Most often, probes have been designed either against 16S or 23S rRNA genes (Ehrmann, Ludwig, & Schleifer, 1994; Hensiek, Krupp, & Stackebrandt, 1992; Hertel et al., 1991; Hertel, Ludwig, Pot, Kersters, & Schleifer, 1993; Sghir, Antonopoulos, & Mackie, 1998), but 16S rRNA based probes remain more popular due to the smaller size of 16S rRNA (1.5 knt) in comparison to the 2.3 knt of the 23S rRNA gene. Species-specific oligonucleotide probes have been used for the successful identification of a number of important Lactobacillus species (Alander et al., 1999; Ampe, 2000; Chagnaud, Machinis, Coutte, Marecat, & Mercenier, 2001; Drake, Small, Spence, & Swanson, 1996; Lucchini et al., 1998; Nakagawa et al., 1994; Park & Itoh, 2005; Pot et al., 1993; Tilsala-Timisjarvi & Alatossava, 1997; Yasui, Okamoto, & Taguchi, 1997).
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2005, FEMS Microbiology LettersMolecular taxonomy and genetics of sourdough lactic acid bacteria
2005, Trends in Food Science and TechnologyIsolation and identification of the dominant Lactobacillus in gut and faeces of pigs using carbohydrate fermentation and 16S rDNA analysis
2005, Journal of Bioscience and BioengineeringMolecular Approaches for the Detection and Identification of Bifidobacteria and Lactobacilli in the Human Gastrointestinal Tract
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Professor Dr. Erko Stackebrandt, Dept. of Microbiology, University of Queensland, St. Lucia, Qld. 4072, Australia