Announcement of population dataDevelopment of the X-linked tetrameric microsatellite markers HumDXS6803 and HumDXS9895 for forensic purpose
Introduction
Nowadays, autosomal short tandem repeat (STR) markers are widely applied to forensic individual identification and paternity testing. However, in some special cases, for example, the alleged father cannot be typed but the relatives of the alleged father can be investigated, or identified whether presumptive half-sister have the same father but the mother cannot be tested, the investigation of gonosomal markers may be more informative than the investigation of autosomal polymorphisms.
Over the past few years, many STR loci on the Y chromosome have been studied [1], [2], [3], [4], [5] but the number of forensically evaluated X-linked STRs offered is very low. To our knowledge, only less than 20 X-linked markers have been described for forensic applications so far [6], [7], [8], [9], [10], [11], [12], [13], [14], [15]. The aim of this paper is to continue studying the STR HumDXS9895, and add the STR HumDXS6803 to the panel of forensically used X markers.
Section snippets
Sample preparation
Blood samples were collected from 292 unrelated healthy blood donors (182 females, 110 males) of Han population living in Wuhan, China. Seventy-eight family trios including female children were checked for regular X-chromosomal inheritance. DNA was isolated using the Chelex-100 method [16].
PCR amplification and STR typing
Primers were synthesized by Sangon Biotech. Co. Ltd. (China) according to the sequence published on GenBank (http://www.gdb.org):DXS6803 5′-GAA ATG TGC TTT GAC AGG AA-3′ 5′-CAA AAA GGG ACA TAT GCT ACT T-3′
Marker information
HumDXS6803 and HumDXS9895 are two unpublished human sequence-tagged site (STS) submitted to the Cooperative Human Linkage Center by J. Murray, V. Sheffield, J.L. Weber, G. Duyk and K.H. Buetow in 1995 (gene bank information). HumDXS6803 is also known as GATA45H11, CHLC.GATA45H11.#T14714 and GDB: G00-365-317 and HumDXS9895 is also known as GATA124B04, CHLC.GATA124B04.T35220. The molecular localization of HumDXS6803 and HumDXS9895 are described with DXS453-DXS3 and pter-DXS207, respectively (gene
Conclusions
The reported population genetic data of the DXS6803 and DXS9895 polymorphisms suggest that these two markers are of interest for forensic analysis. DXS6803 and DXS9895 are suitable for concomitant use in kinship testing without limitations. Further population genetic studies should be conducted with the aim to confirm a low mutation rate. The verification of these results would qualify these two markers as useful tool for solving special cases of kinship testing.
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