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Determination of mimosine and 3,4-dihydroxypyridine in milk and plasma of goats

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Abstract

A simple method for determination of mimosine and 3,4-dihydroxypyridine (3,4-DHP) in plasma and milk was developed. Milk and plasma, with tyrosine as internal standard, were deproteinized using 9% trichloracetic acid and extracted with diethyl ether. Metabolites were separated by isocratic high-performance liquid chromatography, with 0.02 M orthophosphoric acid (pH 2.5) at 0.5 ml/min and a Hypersil ODS microbore column. Mimosine, 3,4-DHP and tyrosine were detected at 275 nm. The recovery of the mimosine added to the plasma samples 101.6±2.3% and 103.3±1.0% for milk samples. 3,4-DHP recovery for plasma samples was 101.2±0.9% and for milk samples 100.8±1.4%. The reproducibility of the method was evaluated by analyzing six plasma samples and six goat milk samples. The analyses yielded relative standard deviations of 2.65 and 2.82%, respectively.

References (5)

  • R. Puchala et al.

    Feed Sci. Technol.

    (1995)
  • B. Tangendjaja et al.

    J. Chromatogr.

    (1983)
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