The effect of zearalenone on some enzymatic parameters in rabbits
Introduction
Zearalenone (F-2 toxin) is a toxin produced by fungi belonging to the genus Fusarium. These fungi contaminate corn, as well as food mixtures for farm animals (Bauer et al., 1980, Dutton and Kinsey, 1996, Molto et al., 1997). The toxin is the main metabolite of secondary metabolism of Fusarium (F.) roseum and F. graminearum (Gedek, 1980), but it was also found in F. culmorum (Jesenská, 1987). Zearalenone belongs to toxins with strong oestrogenic effects (Koch, 1981). There are also data on its capability to induce adverse liver lesions with subsequent development of hepatocarcinoma (National Toxicology Program USA, 1982).
Because fusaria occurred also in grain seeds in Slovakia (Jesenská et al., 1983, Šrobárová, 1997), and because of cereal food mixtures used in some species in animal production, the study of zearalenone's effects on animal health has become topical.
The aim of our work was to study zearalenone effects on the changes in enzymatic activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), and total lactatedehydrogenase (LD) in terms of the response of an organism to damage.
Section snippets
Material and methods
In the experiment, 20 female rabbits (Chinchilla Giganta, drobnochov Košice) were used (average body weight: 3.4±0.5 kg; age: 1 year old). The animals were divided into four groups, each of five animals, and placed into cages. The standard hygiene in the housing corresponded to the principles of The European Convention on Animal Protection (1986). The animals were given a commercial food mixture, KK (Kendice), and drinking water ad libitum. In the experimental group E1, the 10 μg of zearalenone
Results
Table 1 presents activities of AST, ALT, ALP, LD, and GGT recorded at the sampling intervals. In the experimental group E2, significantly higher activities of AST were observed at 168 h compared with both experimental groups C1 (P<0.05) and C2 (P<0.001), and at 336 h with the group C2 (P<0.001). In this group, significant differences (P<0.001) were observed also between the AST activity at first sampling (0 h) and the activities at 168 and 336 h.
Activity of ALT was significantly higher in the
Discussion
Mycotoxins represent one of the negative factors influencing health of animals. The effects of aflatoxins and ochratoxins are quite well known. Recently, intense attention has been paid to toxins of fusaria including zearalenone. Even low doses (1 ppm) of this toxin lead to fertility disorders of swine and cattle (Schweighardt, 1980). Higher doses (50–100 ppm) affect conception, ovulation, implantation, foetal development, and viability of new-borns (Price et al., 1993).
Metabolism of
Acknowledgements
This work was partially supported by VEGA: Grant No. 1/7026/20
References (19)
- et al.
Zearalenone induces modifications of haematological and biochemical parameters in rats
Toxicon
(1996) - et al.
Metabolism of high concentrations of dietary zearalenone by young male turkey poults
Poult. Sci.
(1986) - et al.
Zur Kontamination von Futtermitteln mit toxinbildenden Fusarienstämmen und deren Toxinen
Wien. tieräztl. Mschr.
(1980) - et al.
- et al.
A note on the occurrence of mycotoxins in cereals and animal feedstuffs in Kwazulu Natal, South Africa. South Africa 1984–1993
J. Anim. Sci.
(1996) - et al.
Cited by (105)
Effective degradation of zearalenone by dye-decolorizing peroxidases from Pleurotus ostreatus and its metabolic pathway and toxicity analysis
2024, Science of the Total EnvironmentChronic exposure to zearalenone induces intestinal inflammation and oxidative injury in adult Drosophila melanogaster midgut
2023, Ecotoxicology and Environmental SafetyAntihyperlipidemic potential of diosmin in Swiss Albino mice with high-fat diet induced hyperlipidemia
2021, Saudi Journal of Biological SciencesCitation Excerpt :Meanwhile, the incidence rate in United States is reported to be 75%, which is highest among all chronic liver diseases (El-Sheekh et al., 2014). It has been documented, by earlier studies, that HFD is associated with abnormalities in lipid profile indices and metabolism, and is considered the primary causative factor accountable for the progression to NAFLD (Altunkaynak, 2005; Čonková et al., 2001; Kameshwara et al., 2013). Our results revealed that mice fed with HFD for 90 days exhibited hepatic mutilation, as evidenced by biochemical and histopathology results.
Isolation and characterization of the Bacillus cereus BC7 strain, which is capable of zearalenone removal and intestinal flora modulation in mice
2018, ToxiconCitation Excerpt :Thus, degradation by whole microbe cells or their extracts is the most practical and effective method for ZEN detoxification. ZEN degradation by microorganisms has been explored at the metabolite levels and in terms of enzymatic parameters (Conkova et al., 2001). ZEN can be converted into non- or less-toxic products, such as ZEN-14-sulfate, ZEN-O-14-glucoside, and ZEN-O-16-glucoside, through the reduction of carbonyl ketones, the reduction of double bonds, hydroxylation, methylation, sulfation, or glycosylation.