Developmental study of Müller cells in the rat retina using a new monoclonal antibody, RT10F7
Section snippets
Experimental procedures
All animal experiments were carried out in accordance with the National Institutes of Health guidelines for the care and use of laboratory animals, and all efforts were made to minimize animal suffering and to reduce the number of animals used.
Characterization of the RT10F7 antigen
The antigenic specificity of the RT10F7 mAb was analysed by immunoprecipitation of extracts from retinal cultures labelled metabolically with [35S]methionine. As illustrated in Fig. 1, lane 2, RT10F7 reproducibly immunoprecipitated a band of approximately 36 000 mol. wt. This band was never observed after immunoprecipitation with three other control mAbs. The immunoprecipitate from control mAb RT9F1, which is representative of that seen with the other control mAbs, is shown in Fig. 1, lane 1.
Immunocytochemical localization of the RT10F7 antigen
In
Discussion
In the present study we characterize a new monoclonal antibody RT10F7, which is specific to Müller cells in the retina and recognizes a 36 000 mol. wt antigen. We also provide a detailed profile of immunohistochemically-labelled Müller cells during retinal development.
Conclusions
We have produced and characterized a new monoclonal antibody, RT10F7, that in the retina is specific for Müller glial cells. The antigen, a 36 000 mol. wt protein, was present in the adult retina and throughout development. RT10F7 recognizes Müller cells, even before they have achieved their mature profile in vivo, and delinates the subtle changes that Müller cells undergo with maturation. Its use in cloning studies may eventually allow us to perturb Müller cells to study their function in
Acknowledgements
We thank Dr David Weaver for helpful discussions and Mr Yuan Chang for expert technical assistance. E.N.Y. was supported by a Fogarty International Fellowship, and V.E.K. was supported in part by an award from the Fight for Sight Research Division of the National Society to Prevent Blindness. This work was supported by NIH EY08125.
References (74)
- et al.
Identification and characterization of cell types in monolayer cultures of rat retina using monoclonal antibodies
Brain Res.
(1986) Glial ion channels
Curr. Opin. Neurobiol.
(1991)- et al.
Glial and neuronal forms of the voltage-dependent sodium channel: characteristics and cell-type distribution
Neuron
(1989) - et al.
Ion channel expression by white matter glia: the type-1 astrocyte
Neuron
(1990) - et al.
Immunological, morphological, and electrophysiological variation among retinal ganglion cells purified by panning
Neuron
(1988) - et al.
Immunohistochemical demonstration of glial fibrillary acidic protein in normal rat Müller glia and retinal astrocytes
Neurosci. Lett.
(1985) - et al.
Dendritic maturation in cat retinal ganglion cells: a Lucifer yellow study
Neurosci. Lett.
(1987) - et al.
Organization of radial glia and related cells in the developing murine CNS. An analysis based upon a new monoclonal antibody marker
Neuroscience
(1990) - et al.
Tanycytes: morphology and functions. A review
Int. Rev. Cytol.
(1985) Monoclonal antibodies specific for glia in the chick nervous system
Brain Res.
(1985)
An immunocytochemical study of cat retinal müller cells in culture
Expl Eye Res.
Time-course of stratification of the dendritic fields of ganglion cells in the retina of the cat
Devl Brain Res.
DARPP-32, a dopamine- and cyclic AMP-regulated phosphoprotein in tanycytes of the mediobasal hypothalamus: distribution and relation to dopamine and luteinizing hormone-releasing hormone neurons and other glial elements
Neuroscience
Retinal ganglion cell survival requirements: a major but transient dependence on Müller glia during development
Brain Res.
Guidance of neurons migrating to the fetal monkey neocortex
Brain Res.
Germinal cells in the goldfish retina that produce rod photoreceptors
Devl Biol.
Postnatal development of radial glial (Müller) cells of the rabbit retina
Neurosci. Lett.
Immunocytochemical studies on the development of astrocytes, Müller (glial) cells, and oligodendrocytes in the rabbit retina
Devl Brain Res.
Monoclonal antibodies which recognize different cell types in the rat retina
Nature
Neuronal-glial interactions. A new form of transmission?
Nature
New roles for glia
J. Neurosci.
Ion channels in vertebrate glia
A. Rev. Neurosci.
Proliferation of oligodendrocyte precursor cells depends on electrical activity in axons
Nature
Developmental origin and early differentiation of retinal Muller cells in mice
J. Anat.
The fine structure of the ependymal tanycytes at the level of the arcuate nucleus
J. comp. Neurol.
Endfeet of retinal glial cells have higher densities of ion channels that mediate K+ buffering
Nature
Tanycytes of the third ventricle of the neonatal rat: a Golgi study
Am. J. Anat.
Localization of S-100 protein in Muller cells of the retina – 2. Electron microscopical immunocytochemistry
Invest. Ophthal. vis. Sci.
Postnatal dendritic maturation of alpha and beta ganglion cells in cat retina
J. Neurosci.
Ultrastructure of homografts of the rat median eminence into the anterior chamber of the eye
Neuroendocrinology
Antibodies against filamentous components in discrete cell types of the mouse retina
J. Neurosci.
Muller cell endfeet at the inner surface of the retina: light microscopy
Vis. Neurosci.
Development of synaptic arrays in the inner plexiform layer of neonatal mouse retina
J. comp. Neurol.
Mammalian tanycytes: transmission and scanning electron microscopy
Prog. clin. biol. Res.
Spontaneous impulse activity of rat retinal ganglion cells in prenatal life
Science
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