Protective effects of carnosine against malondialdehyde-induced toxicity towards cultured rat brain endothelial cells

doi:10.1016/S0304-3940(97)00873-2

Neuroscience Letters

Volume 238, Issue 3, 5 December 1997, Pages 135-138

https://doi.org/10.1016/S0304-3940(97)00873-2 Get rights and content

Abstract

Malondialdehyde (MDA) is a deleterious end-product of lipid peroxidation. The naturally-occurring dipeptide carnosine (β-alanyl-l-histidine) is found in brain and innervated tissues at concentrations up to 20 mM. Recent studies have shown that carnosine can protect proteins against cross-linking mediated by aldehyde-containing sugars and glycolytic intermediates. Here we have investigated whether carnosine is protective against malondialdehyde-induced protein damage and cellular toxicity. The results show that carnosine can (1) protect cultured rat brain endothelial cells against MDA-induced toxicity and (2) inhibit MDA-induced protein modification (formation of cross-links and carbonyl groups).

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Acknowledgements

We thank the World Cancer Research Fund and the Age-Concern Institute of Gerontology, King's College London for help with consumables.

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Citation Excerpt :
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Protective effects of carnosine against malondialdehyde-induced toxicity towards cultured rat brain endothelial cells

Abstract

Section snippets

Acknowledgements

FEBS Lett.

Biochem. Biophys. Res. Commun.

Free Radical Biol. Med.

Exp. Cell Res.

FEBS Lett.

Biological significance of histidine-containing dipeptides with special reference to carnosine: chemistry, distribution, metabolism and medical application

Sov. Sci. Rev. D. Physicochem. Biol.

Evaluation of a tetrazolium based semi-automatic colorimetic assay: assessment of radiosensitivity

Cancer Res.

Oxidation of low-density lipoprotein by hypochlorite causes aggregation that is mediated by modification of lysine residues rather than lipid oxidation

Biochem. J.