Contribution to the Study of ATPase Activity in Plasmalemma-enriched Fractions from Jerusalem Artichoke Tubers (Helianthus tuberosus L.) in Relation to their Morphogenetic Properties

doi:10.1016/S0176-1617(86)80097-9

Journal of Plant Physiology

Volume 123, Issue 4, May 1986, Pages 373-380

https://doi.org/10.1016/S0176-1617(86)80097-9 Get rights and content

Summary

Membrane fractions enriched in plasmalemma were prepared from tubers of Jerusalem artichoke. Using acridine orange, evidence for the electrogenic function of the plasmalemma ATPase was obtained. This enzymatic activity is greater in dormant than in non dormant material. This result coroborates the hypothesis that dormancy is an extreme case of correlative inhibition.

References (16)

M.I. De Michelis et al.
Two distinct proton translocating ATPases are present in membranes vesicles from Radish seedlings.
FEBS Letters
(1983)
M. Gendraud et al.
Intracellular comparmentation of ATP in dormant and non dormant tubers of Jerusalem artichoke (Helianthus tuberosus L.) grown in vitro
J. Plant Physiol
(1985)
M. Gogarten-Boekels et al.
Kinetics and specificity of ATP dependant proton translocation measured with acridine orange in microsomal fractions from green suspension cells of Cbenopodium rubrum L
J. Plant Physiol
(1985)
T.S. Seibles
Isolation and electrofocusing of plasma membrane protein from potato tubers
Biochem. Physiol. Pflanzen
(1984)
R. Serrano
Purification and reconstitution of the proton pumping ATPase of fungal and plant plasma membrane.
Archives of Biochemistry and Biophysics
(1983)
K.A. Churchill et al.
Anion-sensitive H⁺ pumping ATPase membrane vesicles from Oat roots
Plant Physiol
(1983)
J.C. Courduroux
Etude du mécanisme physiologique de la tubérisation chez le Topinambour (Helianthus tuberosus L.)
Ann. Sc. Nat. Bot
(1967)
M. Gendraud et al.
Caractéristiques de 1'absorption du saccharose et du tétraphénylphosphonium par les parenchymes de tubercules de Topinambour, dormants et non dormants, cultivés in vitro.
Physiol. Vég.
(1983)

There are more references available in the full text version of this article.

Cited by (17)

Plasma membrane H<sup>+</sup>-ATPase, succinate and isocitrate dehydrogenases activities of vessel-associated cells in walnut trees
2001, Journal of Plant Physiology
In winter and spring, walnut trees exhibit variations of sugar content in the vascular sap. According to their location, the vessel-associated cells (VACs, also called contact cells) could control nutrient exchanges between the storage parenchyma and the xylem vessels. According to the literature, the recovery of sap (influx) occurs at the VAC/vessel interface via an H⁺/sugar symport that depends on the transmembrane pH gradient generated by the plasma membrane H⁺-ATPase. The aim of this study was to investigate the ATPase activity, using a perfusion technique that allows the use of several effectors: carbonyl cyanide m-chlorophenylhydrazone (CCCP) and fusicoccin (FC). During winter, the uncoupler CCCP revealed a low pH gradient between the xylem vessels and the vessel-associated cells. Under these conditions, FC, an activator of the H⁺-ATPase, had no effect on the pH of the perfusion solution, suggesting that the enzyme could be lightly active. In contrast, close to bud break, a high pH gradient was revealed by the use of CCCP, and an acidification of the perfusion solution was observed in the presence of FC. Moreover, cytochemical investigation showed high activity of two respiratory enzymes located in mitochondria: NAD-dependent isocitrate dehydrogenase and succinate dehydrogenase. The hypothesis is that in spring this high respiratory activity of VACs provides a consequent increase in available ATP that can be utilized by the plasma membrane H⁺-ATPase.
Changes in Plasma Membrane Properties of Peach Tree Buds and Stands during Dormancy
1995, Journal of Plant Physiology
Fluidity changes between PM from buds and stands, isolated from dormant, non-dormant and nondormant with an additive cold period peach trees, were monitored by steady-state fluorescence polarization using the lipophilic probe 1,6-diphenyl 1,3,5-hexatriene. The break of dormancy, induced by low temperatures, appeared to be correlated with an increase of the PM fluidity from buds, which became higher than from stands. The corresponding apparent energies of activation, calculated from the Arrhenius plots, were modified with the break of dormancy. Indeed, energies of activation were different between the two territories from dormant material but appeared very close in non-dormant material. A superimposed cold treatment on non-dormant peach tree triggered an enhancement of the fluidity differences between PM from buds and stands. However, the apparent energies of activation between the two territories were not modified. These results were discußed according to the trophic hypothesis.
Localization in Sucrose Gradients of Pyrophosphatase Activities in the Microsomal Fraction of Jerusalem Artichoke (Helianthus tuberosus L.) Tubers
1989, Journal of Plant Physiology
Three distinct Mg²⁺-PPases have been detected in the microsomal vesicles from Jerusalem artichoke. Separation of the different membranes on a linear sucrose gradient showed that these activities were associated with tonoplast (d = 1.11-1.12), Golgi (d = 1.14-1.15) and plasma membrane (d = 1.17-1.18). PPase activity located in the plasmalemma enriched fractions seemed to be different from ATPase activity, considering the effects of the well known effectors of the plasmalemma ATPase type.
Effect of phytohormones on the transmembrane translocation of protons in plasma membrane vesicles from tubers of Solanum tuberosum L.
2000, Membrane and Cell Biology
Changes in parameters of the plasmalemma ATPase during peach vegetative bud dormancy
2000, Biologia Plantarum
Change in plasma membrane ATPase activity during dormancy release of vegetative peach-tree buds
1999, Physiologia Plantarum

View all citing articles on Scopus

View full text

Contribution to the Study of ATPase Activity in Plasmalemma-enriched Fractions from Jerusalem Artichoke Tubers (Helianthus tuberosus L.) in Relation to their Morphogenetic Properties

Summary

FEBS Letters

J. Plant Physiol

J. Plant Physiol

Biochem. Physiol. Pflanzen

Archives of Biochemistry and Biophysics

Anion-sensitive H+ pumping ATPase membrane vesicles from Oat roots

Plant Physiol

Etude du mécanisme physiologique de la tubérisation chez le Topinambour (Helianthus tuberosus L.)

Ann. Sc. Nat. Bot

Caractéristiques de 1'absorption du saccharose et du tétraphénylphosphonium par les parenchymes de tubercules de Topinambour, dormants et non dormants, cultivés in vitro.

Physiol. Vég.

Anion-sensitive H⁺ pumping ATPase membrane vesicles from Oat roots