Elsevier

Immunobiology

Volume 176, Issues 1–2, December 1987, Pages 35-46
Immunobiology

Lysis of natural killer-sensitive and -resistant tumor cells by natural killer cytotoxic factors (NKCF)-containing liposomes

https://doi.org/10.1016/S0171-2985(87)80098-0Get rights and content

Abstract

The lethal hit stage in NK cell-mediated lysis requires a complex series of events involving the release of NKCF, subsequent binding of these factors to the target cell, susceptibility of the target cell to lysis by NKCF.Binding of NKCF alone is not sufficient because a number of tumor cells are able to bind NKCF without being lysed, suggesting the need for an additional processing step active on susceptible target cells.In the present study, we show that the interaction with liposome-incorporated NKCF renders NK resistant target cells sensitive to NKCF-mediated lysis.These results suggest that NKCF may mediate their cytotoxic effects through internalization of these factors into the cytosol.

References (24)

  • L.T. Mimms et al.

    Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucoside

    Biochemistry

    (1981)
  • G. Posts

    The interaction of lipid vesicles (liposomes) with cultured cells and their use as carriers for drugs and macromolecules

  • This work was supported by grant R 93-112 from the Netherlands Organization for the Advancement of Pure Research (R.C.R.) and by grants CA 09120 (S.C.W.and S.S.G.) and CA 35791 (B.B.) from the National Cancer Institute.

    *

    Dr.R.C.Roozemond, Department of Histology and Cell Biology, University of Amsterdam, Academical Medical Center, Meibergdreef 15,1105 AZ Amsterdam, The Netherlands

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