Short communicationConservation of the genomic location of the human serum resistance associated gene in Trypanosoma brucei rhodesiense☆
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Acknowledgements
This work was supported by grants from the UNDP/World Bank/World Health Organization Special Programme for Research and Training in Tropical Diseases and The Wellcome Trust.
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Cited by (15)
Human African Trypanosomiasis: Epidemiology and Control
2006, Advances in ParasitologyCitation Excerpt :The protein encoded by the SRA gene had been identified as responsible for human serum resistance in T. b. rhodesiense (de Greef et al., 1989; de Greef and Hamers, 1994). This target has since been identified as a ubiquitous marker for T. b. rhodesiense throughout eastern Africa, and a further sub-division of these parasites is possible through selective markers for southern and northern isolates (Gibson et al., 2002; Gibson and Ferris, 2003). The specific detection of T. b. gambiense by PCR has focused predominantly on identification of variant specific glycoprotein (VSG) genes, such as LiTat 1.3 and AnTat 11.17 (Magnus et al., 1978; Thi et al., 1991; Bromidge et al., 1993).
Are fitness costs associated with resistance to human serum in Trypanosoma brucei rhodesiense?
2004, Trends in ParasitologyThe trypanosome lytic factor of human serum and the molecular basis of sleeping sickness
2004, International Journal for ParasitologyDetection of Trypanosoma brucei rhodesiense in animals from sleeping sickness foci in East Africa using the serum resistance associated (SRA) gene
2004, Acta TropicaCitation Excerpt :This is an example of correlation between isoenzyme and SRA gene results. However, this may not always be the case, if the SRA gene is susceptible to loss from its telomeric location (Xong et al., 1998; Gibson and Ferris, 2003) by, for example, the recombination events associated with antigenic variation. A possible example of this is the absence of the SRA gene from EATRO 795 and its presence in EATRO 835, two cattle isolates which were identical by isoenzyme analysis (Gibson et al., 1980, 2002); interestingly, EATRO 795 was non-infective to human volunteers (Onyango et al., 1966) and human serum sensitive (Targett and Wilson, 1973), while EATRO 835 infected a volunteer (Onyango et al., 1966).
The Trypanosoma brucei reference strain TREU927/4 contains T. brucei rhodesiense-specific SRA sequences, but displays a distinct phenotype of relative resistance to human serum
2004, Molecular and Biochemical ParasitologyA Primate APOL1 Variant That Kills Trypanosoma brucei gambiense
2016, PLoS Neglected Tropical Diseases
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Note: Nucleotide sequence data reported in this paper are available in the EMBL database under the accession numbers: AJ560640, AJ560641, AJ560642, AJ560643 (downstream region ESAG5 gene from Trypanosoma brucei rhodesiense isolates WB56, KETRI 2291, Gambella II, TRPZ 274, respectively). AJ560644, AJ560645 (partial SRA gene from Trypanosoma brucei rhodesiense isolates WB56, KETRI 2291, respectively).