Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow
Introduction
Mesenchymal stem cells (MSC) isolated from bone marrow (BM-MSC) have been shown to have multi-lineage potential and have been used experimentally in cell-based therapies [1], [2]. Our laboratory has identified a population of putative stem cells in adipose tissue which also have the capacity to differentiate along the adipogenic, osteogenic, chondrogenic, and myogenic lineages [3], [4]. These cells can be isolated from processed lipoaspirate (PLA) and, as such, are termed PLA cells. In this study, we sought to compare the cell surface marker expression of human PLA cells and BM-MSCs. In order to eliminate inter-patient variability, we isolated both cell types from the same donors and cultured them in identical media and conditions.
Section snippets
Materials and methods
All materials were from Sigma unless otherwise stated. All tissue culture plasticware was from Fisher. Fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT). Dulbecco's Modified Media (DMEM), Trypsin/EDTA, and antibiotic-antimycotic (ABAM) were obtained from Cellgro (Hemdon, VA).
Following informed consent, a bone marrow biopsy and excision of a small piece of subcutaneous adipose tissue was performed in five patients undergoing elective hip surgery (HSPC #98-09-007-3).
PLA cells were
Results/discussion
In this study, we compare the cell surface marker expression of culture-expanded cells isolated from the bone marrow (BM-MSC) and adipose tissue (PLA cells) of the same human donors. Passage 4 BM-MSC and PLA cells gave rise to apparently homogenous populations with similar cell size and granularity.
The cell surface marker expression profile of the two cell populations was very similar. Both populations expressed CD13, CD29 (β1 integrin), CD44, CD58, CD90 (Thy-1), CD105 (endoglin), and CD166 [6]
Acknowledgements
The STRO-1 hybridoma developed by Beverly Torok-Storb was obtained from the Developmental Studies Hybridoma Bank maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA 52242. This work was funded in part by the Wunderman Family Foundation, the American Society for Aesthetic Plastic Surgery, the Plastic Surgery Educational Foundation, and the Los Angeles Orthopaedic Hospital Foundation.
References (21)
- et al.
Characterization of cells with osteogenic potential from human marrow
Bone
(1992) - et al.
Integrin-mediated interactions between human bone marrow stromal precursor cells and the extracellular matrix
Bone
(2001) - et al.
The SH-3 and SH-4 antibodies recognize distinct epitopes on CD73 from human mesenchymal stem cells
Biochem. Biophys. Res. Commun.
(2001) - et al.
Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1
Blood
(1991) - et al.
The STRO-1+ fraction of adult human bone marrow contains the osteogenic precursors
Blood
(1994) - et al.
Vascular cell adhesion molecule-1 expressed by bone marrow stromal cells mediates the binding of hematopoietic progenitor cells
Blood
(1992) - et al.
Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo
Biochem. Biophys. Res. Commun.
(2002) - et al.
Multilineage potential of adult human mesenchymal stem cells
Science
(1999) Marrow stromal cells as stem cells for nonhematopoietic tissues
Science
(1997)- et al.
Multi-lineage cells from human adipose tissue: implications for cell-based therapies
Tissue Eng.
(2001)
Cited by (0)
- 1
Fax: +1-310-206-1120.