Elsevier

Immunology Letters

Volume 89, Issues 2–3, 31 October 2003, Pages 267-270
Immunology Letters

Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow

https://doi.org/10.1016/S0165-2478(03)00108-1Get rights and content

Abstract

Our laboratory has characterized a population of stromal cells obtained from adipose tissue termed processed lipoaspirate cells (PLAs). PLAs, like bone-marrow derived mesenchymal stem cells (BM-MSCs), have the capacity to differentiate along the adipogenic, osteogenic, chondrogenic, and myogenic lineages, In order to better characterize these two multi-lineage populations, we examined the surface phenotype of both bone marrow and adipose tissue-derived cells from five patients undergoing surgery. PLA and BM-MSC cells were isolated, subcultivated, and evaluated for cell surface marker expression using flow cytometry. PLA and BM-MSC cells both expressed CD13, CD29, CD44, CD90, CD105, SH-3, and STRO-1. Differences in expression were noted for cell adhesion molecules CD49d (Integrin α4), CD54 (ICAM-1), CD34, and CD106 (VCAM-1). While markedly similar, the surface phenotypes of PLA and BM-MSC cells are distinct for several cell adhesion molecules implicated in hematopoietic stem cell homing, mobilization, and proliferation.

Introduction

Mesenchymal stem cells (MSC) isolated from bone marrow (BM-MSC) have been shown to have multi-lineage potential and have been used experimentally in cell-based therapies [1], [2]. Our laboratory has identified a population of putative stem cells in adipose tissue which also have the capacity to differentiate along the adipogenic, osteogenic, chondrogenic, and myogenic lineages [3], [4]. These cells can be isolated from processed lipoaspirate (PLA) and, as such, are termed PLA cells. In this study, we sought to compare the cell surface marker expression of human PLA cells and BM-MSCs. In order to eliminate inter-patient variability, we isolated both cell types from the same donors and cultured them in identical media and conditions.

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Materials and methods

All materials were from Sigma unless otherwise stated. All tissue culture plasticware was from Fisher. Fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT). Dulbecco's Modified Media (DMEM), Trypsin/EDTA, and antibiotic-antimycotic (ABAM) were obtained from Cellgro (Hemdon, VA).

Following informed consent, a bone marrow biopsy and excision of a small piece of subcutaneous adipose tissue was performed in five patients undergoing elective hip surgery (HSPC #98-09-007-3).

PLA cells were

Results/discussion

In this study, we compare the cell surface marker expression of culture-expanded cells isolated from the bone marrow (BM-MSC) and adipose tissue (PLA cells) of the same human donors. Passage 4 BM-MSC and PLA cells gave rise to apparently homogenous populations with similar cell size and granularity.

The cell surface marker expression profile of the two cell populations was very similar. Both populations expressed CD13, CD29 (β1 integrin), CD44, CD58, CD90 (Thy-1), CD105 (endoglin), and CD166 [6]

Acknowledgements

The STRO-1 hybridoma developed by Beverly Torok-Storb was obtained from the Developmental Studies Hybridoma Bank maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA 52242. This work was funded in part by the Wunderman Family Foundation, the American Society for Aesthetic Plastic Surgery, the Plastic Surgery Educational Foundation, and the Los Angeles Orthopaedic Hospital Foundation.

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