Development and characterization of monoclonal antibodies against canine trypsin

doi:10.1016/S0165-2427(01)00293-8

Veterinary Immunology and Immunopathology

Volume 80, Issues 3–4, 10 August 2001, Pages 333-338

https://doi.org/10.1016/S0165-2427(01)00293-8 Get rights and content

Abstract

Canine cationic trypsin was purified by salting-out, gel filtration and affinity chromatography. Purity was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight was ca. 28 kDa by SDS-PAGE.

Thirty hybridomas were obtained which produced mAb to canine cationic trypsin by the cell fusion technique. Twenty-two of these recognized cationic trypsin only, while eight hybridomas recognized both cationic and anionic trypsin. Several of the anti-canine cationic trypsin mAb were purified by salting-out and DEAE ion-change chromatography using ascites fluid of immunized BALB/c mice. The mAb proved to have very high specificity to canine cationic trypsin as shown by immunoblotting and it may be possible to use them to develop clinical assays.

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Acknowledgements

We thank Onda Masaaki, Yasuhiko Inagaki and Mitsuru Furuichi for technical assistance.

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Short communicationDevelopment and characterization of monoclonal antibodies against canine trypsin

Abstract

Section snippets

Acknowledgements

Biochim. Biophys. Acta

Immunochemistry

Biochim. Biophys. Acta

Biochim. Biophys. Acta

Anal. Biochem.

Clearance of circulating anionic and cationic pancreatic trypsinogens in the rat

Am. J. Physiol.

Monoclonal antibodies to human pancreatic trypsin1 inhibit the activation of human trypsinogens 1 and 2

Eur. J. Biochem.

Two human trypsinogens. Purification, molecular properties, and N-terminal sequences

Biochemistry

Short communication
Development and characterization of monoclonal antibodies against canine trypsin