Cadmium bioconcentration in the echinoid Paracentrotus lividus: Influence of the cadmium concentration in seawater

doi:10.1016/S0141-1136(96)00093-1

Marine Environmental Research

Volume 43, Issue 4, June 1997, Pages 303-314

https://doi.org/10.1016/S0141-1136(96)00093-1 Get rights and content

Abstract

Uptake and loss kinetics of cadmium were studied in Paracentrotus lividus exposed for 24 d to different stable Cd concentrations (0 to 1.14 μg Cd l⁻¹) and ¹⁰⁹Cd tracer added to seawater. The whole-body uptake kinetics were linear over the time course of the experiment. The whole-body ¹⁰⁹Cd concentration factor was independent of the stable Cd concentration in ambient seawater. Bioconcentration of Cd in whole individuals and their body compartments was directly proportional to the Cd concentration added to the seawater. The echinoid digestive tract wall showed the highest degree of Cd uptake. Whole-body loss kinetics were described by a 2-component exponential equation. The loss kinetics were similar for each of the treatments examined. Cadmium was found to display a long biological half-life in echinoid tissues; the major fraction (73 to 85%) of the Cd taken up by echinoids was eliminated following a loss component whose biological half-life was of the order of 1 year, regardless of the absolute Cd concentration accumulated by the echinoid. Paracentrotus lividus is suggested as a valuable biomonitor of Cd, particularly where knowledge of Cd concentration variations over long-term is of interest.

References (39)

P. Benon et al.
Distribution of some heavy metals in the Gulf of Fos
Mar. Pollut. Bull.
(1978)
A. Bohn
Trace metals in fucoid algae and purple sea urchins near a high Arctic lead/zinc ore deposit
Mar. Pollut. Bull.
(1979)
M.H. Depledge et al.
Models of regulation and accumulation of trace metals in marine invertebrates
Comp. Biochem. Physiol.
(1990)
R. Fukai et al.
Copper, zinc and cadmium in coastal waters of the NW Mediterranean
Mar. Pollut. Bull.
(1976)
L. Huynh-Ngoc et al.
Cadmium in the Rhône river
Wat. Res.
(1988)
C.R.C. Sheppard et al.
Pollution of the Mediterranean around Naples
Mar. Pollut. Bull.
(1974)
N. Ablanedo et al.
Evaluaciòn del erizo de mar Echinometra lucunter como indicador de contaminación por metales pesados, Cuba
Aquat. Living Resour.
(1990)
H. Augier et al.
Teneurs en métaux lourds des oursins comestibles Paracentrotus lividus (Lamarck) prélevés dans quatre secteurs tests du littoral de Marseille (Méditerranée France)
Vie Marine
(1989)
J.P. Béthoux et al.
Trace metal pollution in the Mediterranean Sea
Oceanol. Acta
(1990)
P. Bjerregaard
Effect of selenium on cadmium uptake in selected benthic invertebrates
Mar. Ecol. Prog. Ser.
(1988)

J.M. Bouquegneau et al.

The fate of stable pollutants — heavy metals and organochlorines — in marine organisms

J.M. Bouquegneau et al.

Accumulation of technetium by four marine molluscs and transfer to a predatory fish

K.D. Bruland

Trace elements in seawater

G.W. Bryan

Pollution due to heavy metals and their compounds

P.A. Dinnel et al.

A sea urchin test system for marine environmental monitoring

R. Eisler et al.

Cadmium uptake by marine organisms

J. Fish. Res. Bd. Canada

(1972)

S.W. Fowler et al.

Experimental studies on cadmium flux through marine biota

R. Fukai et al.

Tripartite interlaboratory calibration of trace metal measurements on seawater — a summary report

Thalassia Jugoslavica

(1980)

P.J. Hayward et al.

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Cadmium bioconcentration in the echinoid Paracentrotus lividus: Influence of the cadmium concentration in seawater

Abstract

Mar. Pollut. Bull.

Mar. Pollut. Bull.

Comp. Biochem. Physiol.

Mar. Pollut. Bull.

Wat. Res.

Mar. Pollut. Bull.

Evaluaciòn del erizo de mar Echinometra lucunter como indicador de contaminación por metales pesados, Cuba

Aquat. Living Resour.

Teneurs en métaux lourds des oursins comestibles Paracentrotus lividus (Lamarck) prélevés dans quatre secteurs tests du littoral de Marseille (Méditerranée France)

Vie Marine

Trace metal pollution in the Mediterranean Sea

Oceanol. Acta

Effect of selenium on cadmium uptake in selected benthic invertebrates

Mar. Ecol. Prog. Ser.

The fate of stable pollutants — heavy metals and organochlorines — in marine organisms

Accumulation of technetium by four marine molluscs and transfer to a predatory fish

Trace elements in seawater

Pollution due to heavy metals and their compounds

A sea urchin test system for marine environmental monitoring

Cadmium uptake by marine organisms

J. Fish. Res. Bd. Canada

Experimental studies on cadmium flux through marine biota

Tripartite interlaboratory calibration of trace metal measurements on seawater — a summary report

Thalassia Jugoslavica