An in vitro pulp chamber with three-dimensional cell cultures
References (20)
Use of cell cultures for toxicity testing of dental materials—advantages and limitations
J Dent
(1994)- et al.
Regional variability in the permeability of human dentine
Arch Oral Biol
(1987) - et al.
A commercially available cell culture device modified for dentin barrier tests
J Endodon
(1996) - et al.
Characterization, barrier function, and drug metabolism of an in vitro skin model
J Invest Dermatol
(1993) - et al.
The effect of cell monolayer density on the cytotoxicity of metal ions which are released from dental alloys
Dent Mater
(1993) - et al.
Characterization of an in vitro dentin barrier test using a standard toxicant
J Endodon
(1994) - et al.
Comparison of pulpal blood flow in dog canine teeth determined by the laser Doppler and the 133xenon washout methods
Arch Oral Biol
(1990) ANSI-ADA-document no. 41 for Biological Evaluation of cental materials
(1982)ISO 7405: Dentistry—Preclinical evaluation of biocompatibility of medical devices used in dentistry. Test methods for dental materials
(1997)- et al.
New developments in the filter test system for cytotoxicity testing
J Mater Sci Mater Med
(1994)
Cited by (52)
Biocompatibility assessment of resin-based cements on vascularized dentin/pulp tissue-engineered analogues
2021, Dental MaterialsCitation Excerpt :This refers to the pulp analogue within the 24 well plate and the dentin analogue suspended over this pulp analogue within the hanging culture insert, composed of differentiated layer of odontoblast like cells and the treated-dentin disk. Numerous publications have dealt with incorporating the dentin barrier over an artificial pulp chamber in order to simulate the clinical scenario, with several variations, such as utilization of dentin slices of various thicknesses (100, 200, 300, 500, 700 μm) [21,63], cell seeding in 3D polyamide meshes instead of the initial 2D cell cultures on coverslips [21,64–66], using cells of human origin [67,68], and seeding cells in natural hydrogels, such as fibrin and collagen [68]. In general, these devices became more complex and sophisticated through the years, studying the perfusion of eluates from the dentin barrier [20,69], introducing dynamic conditions in the system [23,64,70] and even reproducing the dentin barrier itself [71].
Progress and Challenges in Microengineering the Dental Pulp Vascular Microenvironment
2020, Journal of EndodonticsBiocompatibility of biomaterials – Lessons learned and considerations for the design of novel materials
2017, Dental MaterialsCitation Excerpt :This also means that if the biological properties of novel materials are tested, barriers between materials and target cells should be implemented; in case of filling materials this would be dentin. In order to mimic this situation, we developed the dentin barrier test [40,41], which can be used as an in vitro simulation test for cytotoxicity evaluation of novel dental materials intended to be used for cavity filling. In this test, a split perfusion chamber is divided into two compartments by a defined dentin slice.
In vitro dentin barrier cytotoxicity testing of some dental restorative materials
2017, Journal of DentistryCitation Excerpt :This discrepancy may have arisen because traditional methods combined with two-dimensional (2D) monolayer cell culture behave too sensitively, in comparison to complex in vivo conditions. The dentin barrier test was developed to screen for chemical toxicity to pulp tissue by dental restorative materials, especially those used in direct contact with dentin [13–16]. This method is used instead of traditional in vitro models, as it closely mimics clinical practice and provides results that more accurately reflect in vivo conditions [13,15,16].
Methods to evaluate and strategies to improve the biocompatibility of dental materials and operative techniques
2014, Dental MaterialsCitation Excerpt :It should also be pointed out that a large portion of the in vitro cytotoxicity/biocompatibility studies have been developed with the use of monolayer cell culture models [15,16]. However, the 3D culture models, in which cells are cultivated in specific types of collagen matrix (scaffolds), appear to provide more favorable conditions for the morphological and phenotypical expression of the cells, and this experimental model may also be used for the direct and indirect evaluation of the biologic effects of new dental materials and techniques [24,32]. Another factor that contributes to the efficacy of the in vitro cytotoxicity/biocompatibility tests for dental materials is the selection of the tests to be performed [11], which may vary according to the type of product to be tested and the following levels of response one wishes to obtain: cytotoxicity, the induction of an inflammatory responses, biostimulation or cell differentiation capacity, among other important cellular functions for the homeostasis and repair of the dentin–pulp complex.
- 1
Drs. Schmalz, Schuster, Nuetzel, and Schweikl are affiliated with the Department of Operative Dentistry and Periodontology, University of Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, Germany.