Effects of Abscisic Acid and Water Stress on the Embryo and Plantlet Formation in Anther Culture of Nicotiana tabacum cv. Samsun

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Summary

Effects of short treatments of tobacco anthers with ABA and mannitol on pollen embryogenesis were studied. Three-day treatment with ABA (10−5 M) had a stimulatory effect on the rate of anthers producing plantlets as well as on the number of plantlets formed per anther. Water stress on the anthers, given by adding mannitol (0.5 M) in medium, showed a clear stimulatory effect on plantlet formation when the rate of which in the corresponding control was low. The water stress, imposed on the anthers for 1 day increased the endogenous ABA level of pollen grains from 0.059 ng/grain to 0.120 ng/grain when determined by gas chromatography. Possible roles of ABA and water stress on the pollen embryogenesis were discussed.

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J. Imamura, H. Harada, Institute of Biological Sciences, University of Tsukuba, Sakuramura, Ibaraki-ken, 305 Japan

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