Elsevier

Vision Research

Volume 39, Issue 15, July 1999, Pages 2589-2596
Vision Research

Photoreceptor function of retinal transplants implicated by light-dark shift of S-antigen and rod transducin

https://doi.org/10.1016/S0042-6989(98)00326-5Get rights and content
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Abstract

The aim was to demonstrate functional properties of transplanted histologically normal photoreceptors. Subretinal intact-sheet transplants of fetal E17–E20 rat retinas to light-damaged albino rat eyes were fixed in light or dark, 2 to 42 weeks after transplantation, and stained immunohistochemically for certain phototransduction proteins. In light adapted transplants, transducin was predominantly found in inner segments of parallel-organized photoreceptors. Transducin shifted to the outer segments with dark-adaptation. S-antigen distribution was opposite to transducin. Rhodopsin distribution did not change. The shift of signal transduction proteins correlated to the light conditions indicates that normal phototransduction processes were established in photoreceptors of transplanted retinal sheets.

Keywords

Retinal transplantation
Photoreceptor
Rat
Light damage
Immunohistochemistry
Transduction

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