Polytheonamides, unprecedented highly cytotoxic polypeptides from the marine sponge Theonella swinhoei 2. Structure elucidation☆
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Cited by (55)
Vectorial insertion of a β-helical peptide into membrane: a theoretical study on polytheonamide B
2021, Biophysical JournalCitation Excerpt :Here, we applied the cytotoxic peptide, polytheonamide B (pTB), among other membrane-targeting peptides to understand the dynamic elementary processes of vectorial insertion. The 48-residue pTB is a cytotoxin obtained from the marine sponge Theonella swinhoei (14–23). The potent cytotoxicity of pTB is related to its channel-forming activity across the target cell membrane by spontaneously inserting into the membrane (15,18).
Genome mining methods to discover bioactive natural products
2021, Natural Product ReportsRadical SAM enzymes involved in modifications of RiPP natural products
2020, Comprehensive Natural Products IIIBiotechnological Applications of Bioactive Peptides From Marine Sources
2018, Advances in Microbial PhysiologyCobalamin-Dependent C-Methyltransferases From Marine Microbes: Accessibility via Rhizobia Expression
2018, Methods in EnzymologyCitation Excerpt :Most notably, the cytotoxic membrane-spanning peptides the polytheonamides are posttranslationally tailored with 17 C-methylations at nearly all unactivated sp3 carbon centers (Freeman et al., 2012; Hamada et al., 2010). Isolated from the Japanese sponge Theonella swinhoei (Hamada, Sugawara, Matsunaga, & Fusetani, 1994a, 1994b) and produced by the uncultivated bacterial symbiont “Candidatus Entotheonella factor” (Wilson et al., 2014), polytheonamides were found to be synthesized, in part, by two B12-dependent rSAM C-methyltransferases encoded in their biosynthetic gene cluster (Freeman, Helf, Bhushan, Morinaka, & Piel, 2017). Much like polytheonamides, many putative natural product pathways discovered from either uncultivated or genetically intractable microbes ultimately require heterologous expression to probe the biosynthesis of the encoded metabolite in entirety.
Ribosomally synthesized and post-translationally modified peptide natural product discovery in the genomic era
2017, Current Opinion in Chemical Biology
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Bioactive Marine Metabolites. 56. Part 55: Fusetani, N.; Hamada, T.; Sugawara, T.; Matsunaga, S., a proceeding paper in this issue.