Society of University SurgeonsAnoikis, extracellular matrix, and apoptosis factors in isolated cell transplantation☆,☆☆
Section snippets
Rhesus islet preparation
The rhesus macaque islets were isolated from pancreata removed under clinical conditions of procurement from a heart-beating donor animal. Optimal procurement techniques were used, including retrograde perfusion of the coeliac, superior mesenteric, and renal arteries with University of Wisconsin (UW) preservation fluid and removal and duct cannulation of the donor pancreas. The pancreas was kept at 4°C in iced UW solution. Pancreas digestion was performed by means of a modified Ricordi
Results
Fig 1 shows the initial observation demonstrating a high rate of death in free isolated islets cultured 24 to 48 hours. After culture, the mean viability of the freely isolated islets (EB/AO stain) was strikingly reduced
Discussion
Islet replacement by whole-organ pancreas graft or isolated islet transplantation has been demonstrated to be the therapy of choice for maintaining a constant euglycemic state.19 Unlike whole-organ pancreas grafts done for insulin-dependent diabetes mellitus, isolated islet transplants have a high rate of early (up to 3 months) failure of more than 60%.10 Thus, early donor islet failure is a phenomenon not seen in other whole-organ transplants.
The traumatic nature of islet isolation and the
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Cited by (148)
From islet of Langerhans transplantation to the bioartificial pancreas
2022, Presse MedicaleBioink based on the dECM for 3D bioprinting of bionic tissue, the first results obtained on murine model
2022, BioprintingCitation Excerpt :Initially, beta cells culture constructs was being printed from synthetic materials such as PLGA [16], which were then coated with an ECM-based hydrogel layer containing human pancreatic islets. Increased islet survival in the vicinity of ECM proteins has been demonstrated for decades [17]. Artificial constructs made of synthetic polymer ensure adequate porosity and pore size, and the islets surrounded by proteins such as collagen I, fibronectin and collagen IV were capable of a more effective secretion of insulin than in other systems.
Co-encapsulation of ECM proteins to enhance pancreatic islet cell function
2019, Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas: Volume 2ECM-based scaffolds for pancreas bioengineering
2019, Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas: Volume 2Effectiveness of bioengineered islet cell sheets for the treatment of diabetes mellitus
2018, Journal of Surgical ResearchCitation Excerpt :However, this proved difficult although we tried several different refinement methods. The problem of high cell losses was previously reported by Callewaert et al.28 and might be attributed to the loss of physical interactions during dispersion, which could trigger anoikis, a type of apoptosis.29-33 We conclude that further research is required to find ways to minimize cell losses.
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Supported by National Institutes of Health grants #R01 Al 122293, Al 39793, and Juvenile Diabetes Foundation International grant #198242.
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Reprint requests: Francis T. Thomas, MD, Professor of Surgery, The University of Alabama at Birmingham, Suite 557-A, 1808 7th Avenue, South, Birmingham, AL 35294-0012.