Nucleotide sequence of the lysozyme gene of bacteriophage T4: Analysis of mutations involving repeated sequences*

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The nucleotide sequence of the lysozyme (e) gene of bacteriophage T4 and approximately 130 additional nucleotides on each side has been determined. The 5′-end of the gene for internal protein III appears to be located about 70 base-pairs from the 3′-end of the lysozyme gene. Nucleotide sequence analysis of mutant e genes confirmed that three identified hotspots of frameshift mutations are runs of five A nucleotides in the wild-type gene. The endpoints of two deletions are direct repeats of eight base-pairs in the wild-type gene. Two frameshift mutations with high reversion frequencies are duplications of five or seven base-paris. The cloning and nucleotide sequence determination of the lysozyme gene will facilitate further study of the molecular biology of T4 lysozyme.

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    *

    This research was supported by grants from the National Institutes of Health (GM22731-04 to G. Streisinger and AI13079 and GM26798 to G.R.S.) and from the National Science Foundation (PCM75-18739 to G.R.S.). One of us (G.R.S.) is the recipient of a Research Career Development Award from the National Institutes of Health (AI 00385).

    Present address: Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Wash. 98104, U.S.A.

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