Journal of Molecular Biology
Volume 98, Issue 3, 5 November 1975, Pages 503-508, IN3-IN5, 509-517
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Detection of specific sequences among DNA fragments separated by gel electrophoresis

https://doi.org/10.1016/S0022-2836(75)80083-0Get rights and content

This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters. The fragments can then be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography. The method is illustrated by analyses of restriction fragments complementary to ribosomal RNAs from Escherichia coli and Xenopus laevis, and from several mammals.

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